Brainscape's Knowledge GenomeTM

Browse over 1 million classes created by top students, professors, publishers, and experts.


See full index

VMCB121 (2nd Lab Exam) > Microbial Control > Flashcards

Microbial Control Flashcards

1
Q

It is also known as “gentian violet”

A

Crystal Violet

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
2
Q

Crystal Violet is also known as?

A

“Gentian Violet”

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
3
Q

A triarylmethane dye used for histological and Gram’s staining.

A

Crystal Violet

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
4
Q

Crystal violet is a ____ used for histological ang Gram’s staining

A

Triarylmethane dye

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
5
Q

It possesses anti-microbial effect against certain pathogens

A

Crystal Violet

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
6
Q

Possesses anti-microbial effect against certain pathogens especially in dermatological infections.

A

Crystal Violet

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
7
Q

Crystal Violet possesses anti-microbial effect against certain pathogens especially in?

A

dermatological infections

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
8
Q

It Has been shown to have activity against both Gram positive and Gram negative bacteria

A

Crystal Violet

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
9
Q

A low-cost alternative to treat resistant bacteria in the skin

A

Crystal Violet

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
10
Q

2 Types of Antibiotic Sensitivity Test

A
  1. Kirby-Bauer Method
  2. Minimum Inhibitory Concentration (MIC)
How well did you know this?
1
Not at all
2
3
4
5
Perfectly
11
Q

It is also known as the agar disk diffusion (ADD) test.

A

Kirby-Bauer Method

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
12
Q

Kirby-Bauer Method is also known as the?

A

Agar Disk Diffusion (ADD) test

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
13
Q

It is a standard industrial
procedure for determining whether bacteria are sensitive or resistant to antimicrobial compounds

A

Kirby-Bauer method / Agar Disk Diffusion (ADD) test

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
14
Q

It is when bacteria are placed on a plate of solid growth medium and wafers of antibiotics (white disks, shown) are added to the
plate.

A

Kirby-Bauer Method/ Agar Disk Diffusion (ADD) test

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
15
Q

It determines the lowest
concentration of an antimicrobial agent that prevents a microorganism from
growing visibly in a culture medium.

A

Minimum Inhibitory Concentration (MIC)

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
16
Q

Minimum Inhibitory Concentration uses ____

A

serial dilution of antibiotics

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
17
Q

What uses serial dilution of antibiotics?

A

Minimum Inhibitory Concentration (MIC)

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
18
Q

What agar is used in Kirby-Bauer Disc Diffusion Method?

A

Meuller Hinton Agar

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
19
Q

In the results of Minimum Inhibitory Concentration (MIC), high tubidity means ?

A

Bacterial growth

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
20
Q

In the results of Minimum Inhibitory Concentration (MIC), low tubidity means ?

A

No bacterial growth

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
21
Q

In the results of Minimum Inhibitory Concentration (MIC), it means bacterial growth.

A

High turbidity

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
22
Q

In the results of Minimum Inhibitory Concentration (MIC), it means no bacterial growth.

A

Low turbidity

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
23
Q

The lowest concentration of antimicrobial agent that is capable of inhibiting bacterial growth is called:

A

Minimum Inhibitory Concentration (MIC)

24
Q

It is performed by
soaking stainless steel carriers in bacteria, treating them with the disinfectant, and finally placing the carriers in growth broth
to determine if any still have surviving bacteria

A

Use-Dilution Method

25
Q

What is being soaked in the Use-Dilution Method?

A

Stainless steel carriers

26
Q

Where are stainless steel carriers being soaked in Use-Dilution Method?

A

in bacteria

27
Q

It develops standards, methods, quality control parameters and
interpretive criteria for sensitivity testing.

A

Clinical & Laboratory Standards Institute (CLSI)

28
Q

based on emerging resistance, breakpoints of SIR (susceptible,
intermediate, resistant) can be changed

A

Clinical & Laboratory Standards Institute (CLSI)

29
Q

Clinical & Laboratory Standards Institute (CLSI) based on emerging resistance, ____ (susceptible, intermediate, resistant) can be changed

A

breakpoints of SIR

30
Q

SIR in breakpoints of SIR means?

A
  • Susceptible
  • Intermediate
  • Resistant
31
Q

Approximately 50 antibacterial agents have been tested by them.

A

Clinical & Laboratory Standards Institute (CLSI)

32
Q

How many antibacterial agents have been tested by the Clinical & Laboratory Standards Institute (CLSI)?

A

approximately 50

33
Q

Each laboratory should have a battery of antibiotics used for
testing (least toxic, most effective and clinically important;
refrain from using broad-spectrum, expensive antibiotics)

A

Clinical & Laboratory Standards Institute (CLSI)

34
Q

Battery of antibiotics used for testing

A
  • least toxic
  • most effective
  • clinically important
35
Q

What should be refrained from using in laboratory testing by the Clinical & Laboratory Standards Institute (CLSI)?

A

broad-spectrum, expensive antibiotics

36
Q

interpretive category indicating that the organism is inhibited
by the recommended dose at the infection site

A

Susceptible (S)

37
Q

Organism requires a higher dose of antibiotic for a longer
period of time to be inhibited

A

Intermediate (I)

38
Q

organism is not inhibited by the recommended dose

A

Resistant (R)

39
Q

The term for the Interpretative Standards for Staphylococcus and E. Coli species.

A

Zone Diameter Interpretative Standards

40
Q

Done using McFarland Turbidity
Standard

A

Inoculum standardization

41
Q

Inoculum standardization is done using:

A

McFarland Turbidity
Standard

42
Q

In inoculum standardization, inoculum should be:

A

0.5 MFU (McFarland Units)

43
Q

MFU stands for?

A

McFarland Units

44
Q

Used to standardize the
approximate number of bacteria
in a liquid suspension by visually
comparing the turbidity of a test
suspension with the turbidity of a
McFarland standard

A

Inoculum standardization

45
Q

Composed of barium chloride
and sulfuric acid that are used to
estimate the number of bacteria
in a liquid suspension

A

Inoculum standardization

46
Q

Inoculum standardization is composed of ___ and ___ that are used to estimate the number of bacteria in a liquid suspension

A
  • barium chloride
  • sulfuric acid
47
Q

Other tests that can be used in Antimicrobial Sensitivity Testing and Drug selection

A
  • E-test, “Epsilometer” (Gradient diffusion method)
  • “MIC on a stick”
48
Q

The E in E-test means?

A

Epsilometer

49
Q

Other term for E-test, “Epsilometer”

A

Gradient diffusion method

50
Q

Gradient diffusion method is another term for?

A

E-test, “Epsilometer”

51
Q

Plastic strips impregnated
with antimicrobial on one side
and scale on the other side

A

“MIC on a stick”

52
Q

MIC scale can be viewed where?

A

on other side

53
Q

MIC is read where ___

A

ZOI intersects scale

54
Q

____ is read where ZOI
intersects scale

A

MIC

55
Q

_____ can be viewed on
other side

A

MIC scale

56
Q

Lowest concentration of an antimicrobial agent that visibly inhibits the growth of the organism

A

Minimum Inhibitory Concentration

57
Q

Lowest concentration of the antimicrobial agent that results in the death of the organism

A

Minimum Bactericidal Concentration

VMCB121 (2nd Lab Exam) (6 decks)

Brainscape helps you reach your goals faster, through stronger study habits.
© 2024 Bold Learning Solutions. Terms and Conditions