E10B Flashcards

1
Q

Used to detect the ability of an organism to produce the enzyme Gelatinase (proteolytic) which digest the
gelatin present in the medium.

A

Gelatin Hydrolysis Test

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2
Q

It is a protein from collagen & its hydrolysis indicates
presence of the enzyme

A

Gelatin

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3
Q

Gelatin Hydrolysis indicates presence of?

A

enzyme

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4
Q

In Gelatin Hydrolysis Test, gelatin > ____ > ____

A
  • polypeptides
  • amino acids
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5
Q

In Gelatin Hydrolysis Test, the end-products are used for ?

A

metabolism

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6
Q

In Gelatin Hydrolysis Test, if the result is the medium must stay liquefied even after exposure to cold temperature (4°C), it means the result is?

A

positive

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7
Q

In Gelatin Hydrolysis Test if the result is complete solidification of the medium after exposure
to cold temperature, it means the result is?

A

Negative

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8
Q

Determines the ability of an organism to split amino acid tryptophan to form the compound indole.

A

Indole Test

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9
Q

In Indole test positive resul is

A

Pink colored rink after
addition of Kovac’s Reagent.

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10
Q

What is the Kovac’s Reagent?

A

4 (p)-dimethylamino-benzaldehyde

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11
Q

To determine whether the organism is able to reduce nitrate to
nitrite and subsequently ammonia or reduce it to nitrous acid.

A

Nitrate Reductase Test

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12
Q

To differentiate members of Enterobacteriaceae that produce
enzyme nitrate reductase from those that do not

A

Nitrate Reductase Test

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13
Q

In Nitrate Reductase Test,

(+) sulfanilic acid (A) > ____

A

diazotized sulfanilic acid

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14
Q

In Nitrate Reductase Test,

(+) α-naphthylamine (B) → ____

A

RED azo dye (prontosil)

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15
Q

2 possible reasons for absence of red color in nitrate reduction test:

A
  1. Strain is nitrate-negative
  2. Strain is nitrate-positive
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16
Q

The ammonia produced is used by the cells for the synthesis of
amino acids and other nitrogenous compounds.

A

Assimilation

17
Q

Nitrate or nitrite is used by the organism primarily for energy
metabolism. Nitrate/nitrate serves as the final electron acceptor in
the absence or reduced presence of free oxygen.

A

Dissimilation

18
Q

Certain bacteria convert nitrate to gaseous products like nitrogen and nitrous acid

A

Denitrification

19
Q

This nitrate reacts with the reagent A and B to give red color due to the
formation of ____

20
Q

Color of positive result of Nitrate Reductase Test

21
Q

To check whether the organism can produce urease enzyme for the degradation of urea or not.

A

Urease Test

22
Q

Contents of Urease Test

A
  • Urea
  • Phenol Red (pH indicator)
23
Q

It catalyzes the breakdown of urea into ammonia and carbon dioxide.

24
Q

[Urease Test], With the release of ammonia, the medium becomes alkaline and
changes its color into _____.

25
It is composed of materials most bacteria require for growth
Milk
26
Milk materials required for growth.
- Milk protein casein - sugar lactose - vitamins - minerals - water
27
a pH indicator, is incorporated to determine acid or alkali production, and oxidation and reduction activities
Litmus
28
The characteristic reactions observed of Litmus Milk Test
1. Lactose fermentation 2. Alkaline reaction 3. Curd formation (acid curd and rennin curd) 4. Litmus reduction 5. Proteolysis (peptonization) 6. Gas formation 7. Acid production
29
Litmus milk medium results of no fermentation
A
30
Litmus milk medium results of acid reaction (lactose fermented)
B
31
Litmus milk medium results of curd formation (protein coagulation)
C
32
Litmus milk medium results of clearing of medium (whey formation)
C
33
Litmus milk medium results of cracks in curd (gas formation)
C