E10B Flashcards
Used to detect the ability of an organism to produce the enzyme Gelatinase (proteolytic) which digest the
gelatin present in the medium.
Gelatin Hydrolysis Test
It is a protein from collagen & its hydrolysis indicates
presence of the enzyme
Gelatin
Gelatin Hydrolysis indicates presence of?
enzyme
In Gelatin Hydrolysis Test, gelatin > ____ > ____
- polypeptides
- amino acids
In Gelatin Hydrolysis Test, the end-products are used for ?
metabolism
In Gelatin Hydrolysis Test, if the result is the medium must stay liquefied even after exposure to cold temperature (4°C), it means the result is?
positive
In Gelatin Hydrolysis Test if the result is complete solidification of the medium after exposure
to cold temperature, it means the result is?
Negative
Determines the ability of an organism to split amino acid tryptophan to form the compound indole.
Indole Test
In Indole test positive resul is
Pink colored rink after
addition of Kovac’s Reagent.
What is the Kovac’s Reagent?
4 (p)-dimethylamino-benzaldehyde
To determine whether the organism is able to reduce nitrate to
nitrite and subsequently ammonia or reduce it to nitrous acid.
Nitrate Reductase Test
To differentiate members of Enterobacteriaceae that produce
enzyme nitrate reductase from those that do not
Nitrate Reductase Test
In Nitrate Reductase Test,
(+) sulfanilic acid (A) > ____
diazotized sulfanilic acid
In Nitrate Reductase Test,
(+) α-naphthylamine (B) → ____
RED azo dye (prontosil)
2 possible reasons for absence of red color in nitrate reduction test:
- Strain is nitrate-negative
- Strain is nitrate-positive
