Introduction to Mycology Flashcards
The scientific study of fungi.
Mycology
These are eukaryotic microorganisms.
Fungi
They are larger than bacteria
Fungi
2 Types of Fungi
- Unicellular
- Multicellular
Example of Unicellular Fungi
Yeast
Example of Multicellular Fungi
Molds / filamentous fungi
It is made mostly of polysaccharides.
Fungal cell wall
What is fungal cell wall made of?
Polysaccharides
Polysaccharides that make up the fungal cell wall
- Chitin
- Glucan
- Mannan
Gross Morphology of Fungi
- Pigmentation
- Topography
- Texture
- Size of colonies
Example of Pigmentations of Fungi gross morphology
- tan
- white
- green
- black
It is granular light buff to tan color colony
Trichophyton mentagrophytes
What is the agar used for Trichophyton mentagrophytes?
Sabouraud’s dextrose agar culture
Stain used for Trychophyton mentagrophytes.
Lactophenol cotton blue mount
It has numerous oval to pyriform-shaped microconidia at 400x
Trichophyton mentagrophytes
It looks cottony white to buff color colony.
Microsporum canis
Agar used for Microsporum canis
Sabouraud’s dextrose agar culture
Stain used for Microsporum canis
Lactophenol cotton blue mount
It’s a spindle-shaped macromedia
Microsporum canis
2 Types of hyphae
- septate
- non-septate
It refers to masses of hyphae.
Mycelium
It is commonly seen in yeast.
Pseudohyphae
How does yeast reproduce?
by budding or fission
Conidia can be two types
- macroconidia
- microconidia
Spores found within sac (sporangium)
Endospores
2 types of yeasts
- Capsulated yeast
- Budding yeast
An example of capsulated yeast
Cryptococcus neoformans
What staining is used for Cryptococcus neoformans?
India ink test
It shows germ tubes.
Budding yeast
Budding yeast can show what
germ tubes
Hyphal branches originating in a foot cell in the vegetative mycelium.
Conidiophores
It has conidiophores; hyphal branches originating in a foot cell in the vegetative mycelium and ending in an expanding vesicle.
Aspergillus fumigatus
The staining used for Aspergillus fumigatus.
Lactophenol cotton blue mount
Agar used for Aspergillus fumigatus.
Sabouraud’s dextrose agar culture
Flask-shape layers covering the vesicle of Aspergillus fumigatus.
Phialides
It is where chains of pigmented conidia can be seen.
Phialides
Phialides have chains of pigmented _____ on their ends.
conidia
The asexual reproductive units
Conidia
They give the fungal colony its color.
Conidia (?)
Different types of media
- Non-selective media
- Selective media
- Subculture media
Examples of Non-selective media
- Sabouraud’s dextrose agar
- Brain heart infusion (with or without 5% blood and 1% glucose)
It can be with or without 5% blood and 1% glucose.
Brain heart infusion
Brain heart infusion can be with or without ____ and ____
5% blood
1% glucose
It is with chloramphenicol and cycloheximide,
Mycosel agar
Mycosel agar is with these two.
- Chloramphenicol
- Cycloheximide
Types of Selective media
- Mycosel Agar
- Inhibitory Mold Agar
- Dermatophyte test medium
Types of Subculture media
- Neutral SDA (Emmon’s)
- Cornmeal-Tween 80 agar
- Potato dextrose agar
