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VMCB121 (2nd Lab Exam) > E10A - Physiological Characteristics of Bacteria > Flashcards

E10A - Physiological Characteristics of Bacteria Flashcards

1
Q

It is used to determine the ability of an organism to ferment a given carbohydrate

A

Carbohydrate Fermentation Test

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2
Q

It is used to determine the ability of an organism to degrade amino acids.

A

Carbohydrate Fermentation Test

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3
Q

It is used to determine the ability of an organism to produce gaseous end products in fermentation.

A

Carbohydrate Fermentation Test

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4
Q

Tests under Carbohydrate Metabolism

A
  • Methyl Red-Voges Proskaeur (MRVP) Test
  • Triple Sugar Iron Agar Reaction (TSI)
  • Simmon Citrate Test
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5
Q

It is produced during CHO fermentation

A

acid ± gas

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6
Q

acid ± gas is produced during ______

A

CHO fermentation

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7
Q

Its principle include the production of acid lowers the pH of test medium

A

Carbohydrate Fermentation Test

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8
Q

It is the most common pH indicator used

A

Phenol red

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9
Q

In carbohydrate Fermentation test, the change of test medium color from red to
yellow indicates _____

A

acid formation

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10
Q

It demonstrates gas
formation

A

Inverted Durham tube

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11
Q

What does inverted Durham tube demostrate?

A

gas formation

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12
Q

The are used in microbiology to
detect production of gas by microorganisms.

A

Durham tubes

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13
Q

Composition of Carbohydrate-Fermentation Test

A
  1. Trypticase
  2. Sodium chloride
  3. Phenol red
  4. Carbohydrate
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14
Q

It supports growth of bacteria.

A

Trypticase

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15
Q

It maintains a salt concentration in the medium that is like the cytoplasm of the
microorganisms.

A

Sodium chloride

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16
Q

It’s a pH indicator.

A

phenol red

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17
Q

Carbohydrates included

A
  • Glucose
  • Lactose
  • Sucrose
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18
Q

It changes the medium into YELLOW color.

A

Acid Production (A)

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19
Q

The medium turned darker pink/red color due to protein breakdown without sugar utilization.

A

Alkaline Reaction (ALK)

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20
Q

Presence of SMALL BUBBLES in the inverted Durham TUBE

A

Gas Production (G)

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21
Q

Changes the medium into yellow color along with the presence of small bubbles in the inverted Durham Tube.

A

Acid and Gas Production (AG)

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22
Q

The broth retains the red color.

A

Absence of Fermentation (NG)

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23
Q

It is used to differentiate between coli-aerogenes groups of enteric bacteria.

A

Methyl Red-Voges Proskaeur (MR/VP) test

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24
Q

-Indicates the type of metabolic pathway the organism utilizes with GLUCOSE as the sole source of CARBON for energy.

A

Methyl Red Test

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25
Test of the ability of an organism to produce and maintain stable acid end products from glucose metabolism
Methyl Red Test
26
Detects the presence of acetoin or acetylmethyl carbinol which is an intermediate in the production of butylene glycol.
Voges Proskaeur Test
27
To check whether the microorganism follow 2,3 butanediol production pathway for glucose metabolism or not
Voges Proskaeur Test
28
3 things in the Uninoculated in the MR/VP Test
1. Polypeptone 2. Glucose 3. Dipotassium phosphate
29
Determines whether the bacteria perform mixed acid fermentation when supplied with GLUCOSE
Methyl Red (MR) Test
30
Converts Glucose to 2 pyruvate and then to succinic acid, lactic acid, acetic acid, formic acid, CO2, H2O
Methyl Red (MR) Test
31
In this test, the rationale is the mixed acids produced lower the pH of the medium
Methyl Red (MR) Test
32
In the presence of acids, upon the addition of MR reagent, the color of the broth medium changes to ____
RED
33
In Methyl Red (MR) Test, MR (yellow; pH ____) to MR (red; pH ____)
- 6.0 - 4.4 or less
34
How many drops of MR Reagent?
6 drops
35
In methyl red (MR) test this indicates positive.
Red color
36
The pH indicator in Methyl Red (MR) Test
Methyl Red
37
In MR Test, how many methyl red?
0.1 g in 300 mL of 95% ethyl alcohol.
38
In MR Test, how many distilled water?
200mL
39
Determines the ability of some bacteria to produce 2,3 butanediol from glucose fermentation.
Voges Proskaeur (VP) Test
40
It is not detectable.
2,3-butanediol
41
Since 2,3-butanediol is not detectable what is used instead?
its precursor
42
What test? 2 pyruvate+ NADH --> 2CO2 + 2,3-butanediol
Voges Proskauer (VP) test
43
2,3-butanediol cannot be detected but it does not change the ____
pH of media
44
The precursor of 2,3-butanedoil
Acetylmethyl carbinol or Acetoin
45
What test? Alpha-naphthol + KOH (Potassium hydroxide)
Voges Proskauer (VP) Test
46
Positive Test Result of Voges Proskauer (VP) Test
Pinkish Red Complex
47
Ratio in VP Test
3:1
48
How many drops of Alpha-naphthol in VP test?
6 drops
49
What should be dropped with 6 drops in Voges-Proskauer Test?
Alpha-naphthol
50
The 6 drops of Alpha-naphtol in Voges Proskauer (VP) Test is added to:
40% KOH with creatinine (2 drops)
51
A test which has THREE SUGARS (Lactose, Glucose, Sucrose) and IRON.
Triple Sugar Iron (TSI) test
52
TSI test is a test which has THREE SUGARS (__,___,__) and ___.
- Lactose - Glucose - Sucrose and IRON
53
Composition of TSI test
✔ 0.1% GLUCOSE ✔ 1% LACTOSE ✔ 1% SUCROSE ✔ IRON ✔ PHENOL RED ✔ PEPTONE
54
Inoculate agar by first STABBING through the center of the medium to the bottom of the tube. Then STREAK on the surface of the agar slant. What test?
Triple Sugar Iron (TSI) test
55
In TSI Test after incubation, observe for changes in the medium in _____ for ____ and ____
slant / butt - bubble formation - black ppt
56
TSI Interpretation: no fermentation of sugar; instead, peptones are catabolized.
Alk/ Alk
57
TSI interpretation: fermentation of GLC only
Alk/ A
58
TSI interpretation : fermentation of all three sugar
A/A
59
Catabolism of peptones resulted in the production of what? making the pH alkaline
ammonia
60
Used to determine the ability of an organism: to utilize SODIUM CITRATE as the sole source of CARBON
Simmons Citrate Test
61
Used to determine the ability of an organism: to utilize AMMONIUM as the source of NITROGEN
Simmons Citrate Test
62
Used to differentiate the family Enterobacteriaceae
Simmons Citrate Test
63
Simmons Citrate Test is used to differentiate family ____
Enterobacteriaceae
64
Test to detect the ability of organisms to produce citrase enzyme.
Simmons Citrate Test
65
Composition of Simmons Citrate Test
- Sodium citrate - Ammonium ion - Bromothymol blue
66
In Simmon Citrate Test, The test organism is cultured in a medium which contains ____ and indicator_____.
- sodium citrate - bromothymol blue
67
In Simmon Citrate test, Change in color of indicator from ____ to ____ due to alkaline reaction is indication of citrate utilization by the test organism.
-light green - blue
68
In Simmon Citrate test, Change in color of indicator from light green to blue due to alkaline reaction is indication of ______ by the test organism.
citrate utilization
69
if the organism is able to utilize citrate, it produces ____
citrase enzyme
70
In Simmon Citrate Test, oxaloacetic acid is converted to ____
pyruvate
71
In Simmon Citrate test acetic acid is converted to
CO2
72
In Simmon Citrate Test, CO2 reacts with H2O and NA+ to produce ___
sodium carbonate
73
CARBOHYDRATE FERMENTATION TEST Bacillus subtilis - Glucose = ?
A
74
CARBOHYDRATE FERMENTATION TEST Bacillus subtilis - Lactose = ?
ALK
75
CARBOHYDRATE FERMENTATION TEST Bacillus subtilis - Sucrose = ?
A
76
CARBOHYDRATE FERMENTATION TEST Escherichia coli - Glucose = ?
AG
77
CARBOHYDRATE FERMENTATION TEST Escherichia coli - Lactose = ?
A
78
CARBOHYDRATE FERMENTATION TEST Escherichia coli - Sucrose = ?
AG or ALK
79
CARBOHYDRATE FERMENTATION TEST Proteus mirabilis - Glucose = ?
AG
80
CARBOHYDRATE FERMENTATION TEST Proteus mirabilis - Lactose = ?
ALK
81
CARBOHYDRATE FERMENTATION TEST Proteus mirabilis - Sucrose = ?
ALK
82
MR Test result of E.coli
(+)
83
VP Test result of E.coli
(-)
84
MR Test result of Enterobacter aerogenes
(-)
85
VP Test result of Enterobacter aerogenes
(+)
86
TSI Test E. coli - slant =?
A
87
TSI Test E. coli - Butt/ Tube Bottom =?
A
88
TSI Test E. coli - Gas Production =?
(+)
89
TSI Test E. coli - H2S Production =?
(-)
90
TSI Test E.coli - recorded as?
A/AG
91
TSI Test Proteus mirabilis - slant =?
ALK
92
TSI Test Proteus mirabilis - butt (tube bottom) =?
A
93
TSI Test Proteus mirabilis - gas prod =?
(+)
94
TSI Test Proteus mirabilis - H2S prod =?
(+)
95
TSI Test Proteus mirabilis - recorded as=?
ALK/ AG, H2S
96
TSI Test Pseudomonas aeruginosa - slant =?
ALK
97
TSI Test Pseudomonas aeruginosa - butt (tube bottom) =?
ALK
98
TSI Test Pseudomonas aeruginosa - gas prod =?
(-)
99
TSI Test Pseudomonas aeruginosa - (H2S prod =?
(-)
100
TSI Test Pseudomonas aeruginosa - recorded as =?
ALK/ ALK
101
TSI Test Salmonella sp. - slant =?
ALK
102
TSI Test Salmonella sp. - butt (tube bottom) =?
A
103
TSI Test Salmonella sp. - gas prod =?
(+)
104
TSI Test Salmonella sp. - H2S prod =?
(+)
105
TSI Test Salmonella sp. - recorded as =?
ALK/AG, H2S
106
Simmon Citrate Test E.coli = ?
(-)
107
Simmon Citrate Test Enterobacter aerogenes = ?
(+)

VMCB121 (2nd Lab Exam) (6 decks)

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