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VMCB121 (2nd Lab Exam) > E10A - Physiological Characteristics of Bacteria > Flashcards

E10A - Physiological Characteristics of Bacteria Flashcards

1
Q

It is used to determine the ability of an organism to ferment a given carbohydrate

A

Carbohydrate Fermentation Test

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2
Q

It is used to determine the ability of an organism to degrade amino acids.

A

Carbohydrate Fermentation Test

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3
Q

It is used to determine the ability of an organism to produce gaseous end products in fermentation.

A

Carbohydrate Fermentation Test

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4
Q

Tests under Carbohydrate Metabolism

A
  • Methyl Red-Voges Proskaeur (MRVP) Test
  • Triple Sugar Iron Agar Reaction (TSI)
  • Simmon Citrate Test
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5
Q

It is produced during CHO fermentation

A

acid ± gas

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6
Q

acid ± gas is produced during ______

A

CHO fermentation

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7
Q

Its principle include the production of acid lowers the pH of test medium

A

Carbohydrate Fermentation Test

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8
Q

It is the most common pH indicator used

A

Phenol red

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9
Q

In carbohydrate Fermentation test, the change of test medium color from red to
yellow indicates _____

A

acid formation

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10
Q

It demonstrates gas
formation

A

Inverted Durham tube

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11
Q

What does inverted Durham tube demostrate?

A

gas formation

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12
Q

The are used in microbiology to
detect production of gas by microorganisms.

A

Durham tubes

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13
Q

Composition of Carbohydrate-Fermentation Test

A
  1. Trypticase
  2. Sodium chloride
  3. Phenol red
  4. Carbohydrate
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14
Q

It supports growth of bacteria.

A

Trypticase

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15
Q

It maintains a salt concentration in the medium that is like the cytoplasm of the
microorganisms.

A

Sodium chloride

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16
Q

It’s a pH indicator.

A

phenol red

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17
Q

Carbohydrates included

A
  • Glucose
  • Lactose
  • Sucrose
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18
Q

It changes the medium into YELLOW color.

A

Acid Production (A)

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19
Q

The medium turned darker pink/red color due to protein breakdown without sugar utilization.

A

Alkaline Reaction (ALK)

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20
Q

Presence of SMALL BUBBLES in the inverted Durham TUBE

A

Gas Production (G)

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21
Q

Changes the medium into yellow color along with the presence of small bubbles in the inverted Durham Tube.

A

Acid and Gas Production (AG)

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22
Q

The broth retains the red color.

A

Absence of Fermentation (NG)

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23
Q

It is used to differentiate between coli-aerogenes groups of enteric bacteria.

A

Methyl Red-Voges Proskaeur (MR/VP) test

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24
Q

-Indicates the type of metabolic pathway the organism utilizes with GLUCOSE as the sole source of CARBON for energy.

A

Methyl Red Test

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25
Q

Test of the ability of an organism to produce and maintain stable acid end
products from glucose metabolism

A

Methyl Red Test

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26
Q

Detects the presence of
acetoin or acetylmethyl
carbinol which is an
intermediate in the
production of butylene glycol.

A

Voges Proskaeur Test

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27
Q

To check whether the microorganism follow 2,3 butanediol production pathway for glucose metabolism or not

A

Voges Proskaeur Test

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28
Q

3 things in the Uninoculated in the MR/VP Test

A
  1. Polypeptone
  2. Glucose
  3. Dipotassium phosphate
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29
Q

Determines whether the bacteria perform mixed acid fermentation when supplied with GLUCOSE

A

Methyl Red (MR) Test

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30
Q

Converts Glucose to 2 pyruvate and then to succinic acid, lactic acid, acetic acid, formic acid, CO2, H2O

A

Methyl Red (MR) Test

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31
Q

In this test, the rationale is the mixed acids produced lower the pH of the medium

A

Methyl Red (MR) Test

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32
Q

In the presence of acids, upon the addition of MR reagent, the color of the broth medium changes to ____

A

RED

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33
Q

In Methyl Red (MR) Test, MR (yellow; pH ____) to MR (red; pH ____)

A
  • 6.0
  • 4.4 or less
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34
Q

How many drops of MR Reagent?

A

6 drops

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35
Q

In methyl red (MR) test this indicates positive.

A

Red color

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36
Q

The pH indicator in Methyl Red (MR) Test

A

Methyl Red

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37
Q

In MR Test, how many methyl red?

A

0.1 g in 300 mL of 95% ethyl alcohol.

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38
Q

In MR Test, how many distilled water?

A

200mL

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39
Q

Determines the ability of some bacteria to produce 2,3 butanediol from glucose fermentation.

A

Voges Proskaeur (VP) Test

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40
Q

It is not detectable.

A

2,3-butanediol

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41
Q

Since 2,3-butanediol is not detectable what is used instead?

A

its precursor

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42
Q

What test?

2 pyruvate+ NADH –> 2CO2 + 2,3-butanediol

A

Voges Proskauer (VP) test

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43
Q

2,3-butanediol cannot be detected but it does not change the ____

A

pH of media

44
Q

The precursor of 2,3-butanedoil

A

Acetylmethyl carbinol or Acetoin

45
Q

What test?

Alpha-naphthol + KOH (Potassium hydroxide)

A

Voges Proskauer (VP) Test

46
Q

Positive Test Result of Voges Proskauer (VP) Test

A

Pinkish Red Complex

47
Q

Ratio in VP Test

A

3:1

48
Q

How many drops of Alpha-naphthol in VP test?

A

6 drops

49
Q

What should be dropped with 6 drops in Voges-Proskauer Test?

A

Alpha-naphthol

50
Q

The 6 drops of Alpha-naphtol in Voges Proskauer (VP) Test is added to:

A

40% KOH with creatinine (2 drops)

51
Q

A test which has THREE
SUGARS (Lactose, Glucose,
Sucrose) and IRON.

A

Triple Sugar Iron (TSI) test

52
Q

TSI test is a test which has THREE
SUGARS (__,___,__) and ___.

A
  • Lactose
  • Glucose
  • Sucrose

and IRON

53
Q

Composition of TSI test

A

✔ 0.1% GLUCOSE
✔ 1% LACTOSE
✔ 1% SUCROSE
✔ IRON
✔ PHENOL RED
✔ PEPTONE

54
Q

Inoculate agar by
first STABBING through
the center of the medium
to the bottom of the tube.
Then STREAK on the
surface of the agar slant.

What test?

A

Triple Sugar Iron (TSI) test

55
Q

In TSI Test after incubation, observe for changes in the medium in _____ for ____ and ____

A

slant / butt

  • bubble formation
  • black ppt
56
Q

TSI Interpretation: no fermentation of sugar; instead, peptones are catabolized.

A

Alk/ Alk

57
Q

TSI interpretation: fermentation of GLC only

A

Alk/ A

58
Q

TSI interpretation : fermentation of all three sugar

A

A/A

59
Q

Catabolism of peptones resulted in the production of what? making the pH alkaline

A

ammonia

60
Q

Used to determine the ability of an organism: to utilize SODIUM CITRATE as the sole source of CARBON

A

Simmons Citrate Test

61
Q

Used to determine the ability of an organism: to utilize AMMONIUM as the source of NITROGEN

A

Simmons Citrate Test

62
Q

Used to differentiate the
family Enterobacteriaceae

A

Simmons Citrate Test

63
Q

Simmons Citrate Test is used to differentiate family ____

A

Enterobacteriaceae

64
Q

Test to detect the ability of
organisms to produce citrase
enzyme.

A

Simmons Citrate Test

65
Q

Composition of Simmons Citrate Test

A
  • Sodium citrate
  • Ammonium ion
  • Bromothymol blue
66
Q

In Simmon Citrate Test, The test organism is cultured in a medium which contains ____ and indicator_____.

A
  • sodium citrate
  • bromothymol blue
67
Q

In Simmon Citrate test, Change in color of indicator from ____ to ____ due to alkaline
reaction is indication of citrate utilization by the test organism.

A

-light green
- blue

68
Q

In Simmon Citrate test, Change in color of indicator from light green to blue due to alkaline
reaction is indication of ______ by the test organism.

A

citrate utilization

69
Q

if the organism is able to utilize citrate, it produces ____

A

citrase enzyme

70
Q

In Simmon Citrate Test, oxaloacetic acid is converted to ____

A

pyruvate

71
Q

In Simmon Citrate test acetic acid is converted to

A

CO2

72
Q

In Simmon Citrate Test, CO2 reacts with H2O and NA+ to produce ___

A

sodium carbonate

73
Q

CARBOHYDRATE FERMENTATION TEST

Bacillus subtilis - Glucose = ?

A

A

74
Q

CARBOHYDRATE FERMENTATION TEST

Bacillus subtilis - Lactose = ?

A

ALK

75
Q

CARBOHYDRATE FERMENTATION TEST

Bacillus subtilis - Sucrose = ?

A

A

76
Q

CARBOHYDRATE FERMENTATION TEST

Escherichia coli - Glucose = ?

A

AG

77
Q

CARBOHYDRATE FERMENTATION TEST

Escherichia coli - Lactose = ?

A

A

78
Q

CARBOHYDRATE FERMENTATION TEST

Escherichia coli - Sucrose = ?

A

AG or ALK

79
Q

CARBOHYDRATE FERMENTATION TEST

Proteus mirabilis - Glucose = ?

A

AG

80
Q

CARBOHYDRATE FERMENTATION TEST

Proteus mirabilis - Lactose = ?

A

ALK

81
Q

CARBOHYDRATE FERMENTATION TEST

Proteus mirabilis - Sucrose = ?

A

ALK

82
Q

MR Test result of E.coli

A

(+)

83
Q

VP Test result of E.coli

A

(-)

84
Q

MR Test result of Enterobacter aerogenes

A

(-)

85
Q

VP Test result of Enterobacter aerogenes

A

(+)

86
Q

TSI Test

E. coli - slant =?

A

A

87
Q

TSI Test

E. coli - Butt/ Tube Bottom =?

A

A

88
Q

TSI Test

E. coli - Gas Production =?

A

(+)

89
Q

TSI Test

E. coli - H2S Production =?

A

(-)

90
Q

TSI Test

E.coli - recorded as?

A

A/AG

91
Q

TSI Test

Proteus mirabilis - slant =?

A

ALK

92
Q

TSI Test

Proteus mirabilis - butt (tube bottom) =?

A

A

93
Q

TSI Test

Proteus mirabilis - gas prod =?

A

(+)

94
Q

TSI Test

Proteus mirabilis - H2S prod =?

A

(+)

95
Q

TSI Test

Proteus mirabilis - recorded as=?

A

ALK/ AG, H2S

96
Q

TSI Test

Pseudomonas aeruginosa - slant =?

A

ALK

97
Q

TSI Test

Pseudomonas aeruginosa - butt (tube bottom) =?

A

ALK

98
Q

TSI Test

Pseudomonas aeruginosa - gas prod =?

A

(-)

99
Q

TSI Test

Pseudomonas aeruginosa - (H2S prod =?

A

(-)

100
Q

TSI Test

Pseudomonas aeruginosa - recorded as =?

A

ALK/ ALK

101
Q

TSI Test

Salmonella sp. - slant =?

A

ALK

102
Q

TSI Test

Salmonella sp. - butt (tube bottom) =?

A

A

103
Q

TSI Test

Salmonella sp. - gas prod =?

A

(+)

104
Q

TSI Test

Salmonella sp. - H2S prod =?

A

(+)

105
Q

TSI Test

Salmonella sp. - recorded as =?

A

ALK/AG, H2S

106
Q

Simmon Citrate Test

E.coli = ?

A

(-)

107
Q

Simmon Citrate Test

Enterobacter aerogenes = ?

A

(+)

VMCB121 (2nd Lab Exam) (6 decks)

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