Microarray Technology

Question 1

What does it enable

5.4.4

Answer 1

Rapid gene expression profiling or gene sequence variation of thousands of jeans in an individual

Question 2

What does it involve

5.4.4

Answer 2

DNA bound (hybridised) to a microarray chip

Question 3

What does it bind

5.4.4

Answer 3

It binds the sample DNA to a probe on the chip which can produce a signal that can be fluorescent or chemiluminescent

Question 4

I hat does it digitally analyses

5.4.4

Answer 4

It digitally analyses the chip to calculate the strength of the signal produced at each spot, with the strength of the signal representing the level of gene expression in the individual

Question 5

What is a chemiluminesecent tag?

Answer 5

I can aluminous and tie involves omission of light as a consequence of a particular reaction (a reaction in the hybridisation process)

Question 6

Usesnif microarrays

Answer 6

They can identify mutations, SNPs or provide information on the expression level of genes.

Question 7

Why is Michael away technology not gene sequencing

Answer 7

It can compare how sample DNA is different from normal DNA and compare the expression of genes between different groups

Question 8

What do microarray chips consist of

Answer 8

A solid base such as glass or silicone onto which is attached a grid of thousands of microscopic spots or Wales. To each of the sports is added a different sequence of DNA, each of which can act as a DNA probe. Typically each board contains multiple copies of the identical DNA sequence.