Micro - Vibrio & Related Genera

Question 1

Common characteristics of Genus Vibrio

Answer 1

• Gram -
• Oxidase +
• Curved bacilli
• Most species are soonoses
• Most are marine
• Some in fresh water

Question 2

What species of genus Vibrio causes the ds. Cholerae?

Answer 2

Vibrio cholerae

Question 3

What type of ds. is Cholera?

Answer 3

Enteric ds. - massive amounts of fluids lost

Question 4

How is Vibrio cholerae spread?

Answer 4

Water bourne

Question 5

What are the virulence factors of Vibrio cholerae?

Answer 5

• Exotoxin
• Flagellum

Question 6

What is unique about the exotoxin in Vibrio cholerae?

Answer 6

Exotoxin has ADP ribosylating activity

Question 7

What is affected in the GI tract when Vibrio cholerae is present?

Answer 7

The fluid secreting proteins in the GI tract are affected

Question 8

What is a symptom of Cholera ds.?

Answer 8

“rice water feces”

Question 9

What other organism has an exotoxin that has ADP ribosylating activity and affects the fluid secreting proteins of the GI tract?

Answer 9

E.coli

Question 10

How would you differentiate Vibrio cholerae from E.coli?

Answer 10

V.cholerae:

• shape - curved bacilli
• oxidase +

E.coli

• bacilli
• oxidase -

Question 11

To contract Cholera does it require a large or small does of the bacteria Vibrio cholerae?

Answer 11

Large infectious dose

Question 12

Is the ds. Cholera considered an infection or intoxication?

Answer 12

food/water INFECTION

Question 13

What is the incubation period for Cholera ds.?

Answer 13

24-48 hrs.

Question 14

What symptoms will you see with Cholera ds.?

Answer 14

• Massive fluid loss
• Dehydration
• Electolyte imbalance
• “RICE WATER FECES”

Question 15

What species of the of the Vibrio genus causes a food INFECTION associated with raw seafood/fish

Answer 15

Vibrio parahaemolyticus

Question 16

What symptoms will you see with the food infection caused by Vibrio parahaemolyticus?

Answer 16

• Gastroenteritits
• Mild diarrhea

Question 17

Does Vibrio parahaemolyticus replicate fast or slow?

Answer 17

Rapid - replicates every 9 min

Question 18

What species of the Vibrio genus causes a flesh eating ds.?

Answer 18

Vibrio vulnificus

Question 19

Where is Vibrio vulnificus found?

Answer 19

Marine water (common in Gulf of Mexico)

Question 20

How does Vibrio vulnificus spread?

Answer 20

Enters through cuts/scrapes on skin

Question 21

Vibrio vulnificus is consdiered what kind of bacteria?

Answer 21

Flesh eating bacteria

Question 22

What are the 4 flesh eating bacteria?

Answer 22

Gram +

• Streptococcus pyogenes
• Staphylocococcus aureus

Gram -

• Vibrio vulnificus
• Aeromonas species

Question 23

What organism is responsible for a food INFECTION associated with raw eggs, raw chicken and hamburger?

Answer 23

Campylobacter jejuni

Question 24

What is Campylobacter jejuni also responsible for?

Answer 24

3rd most common cause of gasterenteritis

Question 25

What is more common Campylobacter jejuni or Salmonella enteritidis?

Answer 25

Campylobacter jejuni

Question 26

How could you differentiate Campylobacter jejuni from Salmonella enteritidis and E.coli?

Answer 26

Campylobacter jejuni

• shape - curved bacilli
• oxidase +

Salmonella enteritids

• Shape - bacilli
• oxidase -

E.coli

• Shape - bacilli
• oxidase -

Question 27

Which organism causes 90% of all gastric ulcers?

Answer 27

Helicobacter pylori

Question 28

Helicobacter pylori is what type of flora?

Answer 28

Normal flora in the stomach

Question 29

What is unique about Helicobacter pylori?

Answer 29

It contains a potent urease that protects the organism and allows for ID of infection