Micro special culture requirements/Stains Flashcards by Ellen Pigott

H. influenzae

Chocolate agar with factors V and X

How well did you know this?

Not at all

Perfectly

N. gonorrhae, N. meningitidis

Thayer-Martin (VPN) media

How well did you know this?

Not at all

Perfectly

B. pertussis

Bordet-Gengou (potato) agar

How well did you know this?

Not at all

Perfectly

C. diphtheriae

Tellurite agar, Loffler medium

How well did you know this?

Not at all

Perfectly

M. tuberculosis

Lowenstein-Jensen agar

How well did you know this?

Not at all

Perfectly

M. pneumoniae

Eaton agar (requires cholesterol)

How well did you know this?

Not at all

Perfectly

Lactose-fermenting enterics

Pink on MacConkey agar

How well did you know this?

Not at all

Perfectly

E.coli

MacConkey agar or Eosin-Methylene Blue (EMB) agar

How well did you know this?

Not at all

Perfectly

Legionella

Silver stain; charcoal yeast extract supplemented with cysteine and iron

How well did you know this?

Not at all

Perfectly

Fungi

Silver stain; sabouraud agar

How well did you know this?

Not at all

Perfectly

Tropheryma whipplei

Periodic acid-Schiff (PAS): stains glycogen and mucopolysaccharides

How well did you know this?

Not at all

Perfectly

Chlamydia

Giemsa stain

How well did you know this?

Not at all

Perfectly

Borrelia

Giemsa stain

How well did you know this?

Not at all

Perfectly

Rickettsiae

Giemsa stain

How well did you know this?

Not at all

Perfectly

Trypanosomes

Giemsa stain

How well did you know this?

Not at all

Perfectly

Plasmodium

Giemsa stain

Acid-fast organisms (Nocardia, mycobacterium)

Ziehl-Neelsen (carbol fuchsin)

Cryptococcus neoformans

India ink

H. pylori

Silver stain

Bacteria that can’t Gram stain

Treponema
Mycobacteria
Mycoplasma
Legionella, Rickettsia, Chlamydia (IC) 
Treponemes

Treponemes

Dark field microscopy and fluorescent Ab staining