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First Aid > Micro special culture requirements/Stains > Flashcards

Micro special culture requirements/Stains Flashcards

1
Q

H. influenzae

A

Chocolate agar with factors V and X

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2
Q

N. gonorrhae, N. meningitidis

A

Thayer-Martin (VPN) media

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3
Q

B. pertussis

A

Bordet-Gengou (potato) agar

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4
Q

C. diphtheriae

A

Tellurite agar, Loffler medium

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5
Q

M. tuberculosis

A

Lowenstein-Jensen agar

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6
Q

M. pneumoniae

A

Eaton agar (requires cholesterol)

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7
Q

Lactose-fermenting enterics

A

Pink on MacConkey agar

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8
Q

E.coli

A

MacConkey agar or Eosin-Methylene Blue (EMB) agar

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9
Q

Legionella

A

Silver stain; charcoal yeast extract supplemented with cysteine and iron

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10
Q

Fungi

A

Silver stain; sabouraud agar

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11
Q

Tropheryma whipplei

A

Periodic acid-Schiff (PAS): stains glycogen and mucopolysaccharides

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12
Q

Chlamydia

A

Giemsa stain

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13
Q

Borrelia

A

Giemsa stain

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14
Q

Rickettsiae

A

Giemsa stain

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15
Q

Trypanosomes

A

Giemsa stain

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16
Q

Plasmodium

A

Giemsa stain

17
Q

Acid-fast organisms (Nocardia, mycobacterium)

A

Ziehl-Neelsen (carbol fuchsin)

18
Q

Cryptococcus neoformans

A

India ink

19
Q

H. pylori

A

Silver stain

20
Q

Bacteria that can’t Gram stain

A 
Treponema
Mycobacteria
Mycoplasma
Legionella, Rickettsia, Chlamydia (IC) 
Treponemes
21
Q

Treponemes

A

Dark field microscopy and fluorescent Ab staining

First Aid (18 decks)

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