Micro (NOT PATHOMA)

Question 1

Gram + Coccus (2)

Answer 1

Staphylococcus; Streptococcus

Question 2

Gram - Coccus

Answer 2

Neisseria

Question 3

Gram + Rod (Bacillus) [6]

Answer 3

Clostridium; Corynebacterium; Bacillus; Listeria; Mycobacterium (Acid fast); Gardnerella (Gram variable)

Question 4

No cell wall - no gram stain

Answer 4

Mycoplasma. Contain sterols

Question 5

Branching filamentous Gram +[2]

Answer 5

Actinomyces; Nocardia

Question 6

Bug found on silver stain

Answer 6

Legionella; Fungi; H. pylori

Question 7

Giemsa stain [5]

Answer 7

Certain Bugs Really Try my Patience: Chylamydia; Borrelia; Rickettsiae; Trypanosomes; Plasmodium

Question 8

Ziehl-Beelsen (Carbol Fuchsi) stain

Answer 8

Acid fast organisms - Nocardia, Mycobacterium

Question 9

Chocolate agar with factor V (NAD+) and X (hematin)

Answer 9

H. influenzae

Question 10

Components [3] of Thayer-Martin/VPN media

Answer 10

Vancomycin - inhibits gram +; Polymyxin - inhibit gram - except Neisseria; Nystatin - inhibits fungi

Question 11

Thayer-martin agar [2]

Answer 11

N. gonorrhoeae; N meningitidis

Question 12

Bordet-Gengou )potato) agar

Answer 12

B. pertussis

Question 13

Tellurite plate; Loffler’s media

Answer 13

C. diphtheriae

Question 14

Lowenstein-Jensen agar

Answer 14

M. tuberculosis

Question 15

Eaton’s agar

Answer 15

M. pneumoniae

Question 16

Sabouraud’s agar

Answer 16

Fungi

Question 17

Charcoal yeast extract with cysteiene and Fe

Answer 17

Legionella

Question 18

Catalase + Organisms [7]

Answer 18

You need PLACESS for you CATs.

Pseudomonas; Listeria; Aspergillus; Candida; E.coli; S. aureus; Serratia

Question 19

Urease-positive bugs

Answer 19

“CHuck Norris hates PUNKSS”;
Cryptococcus; H. pylori; Proteus; Ureaplasma; Nocardia; Klebsiella; S. epidermidis; S. saprophyticus

Question 20

S. aureus virulence factor. Binds Fc region of Ig. Prevents opsonization and phagocytosis.

Answer 20

Protein A

Question 21

Virulence factor secreted by S. pneumonia; H. influenza type B; and Neisseria

Answer 21

IgA protease

Question 22

Group A strep virulence factor that prevents phagocytosis

Answer 22

M protein

Question 23

Labs; staph vs strep

Answer 23

Catalase: (Staph catalase +)

Question 24

Labs: Differentiate types of Staph

Answer 24

Coagulase: S. aureus = Coagulase +;

Novobiocin sensitive: S. epidermidis; Novobiocin resistant: S. saprophyticus

Question 25

Gram + cocci; catalase -; alpha hemolytic; Optochin sensitive

Answer 25

S. pneumoniae

Question 26

Gram + Cocci; catalase -; alpha hemolytic; optochin resistant

Answer 26

Viridans strep (S. mutans)

Question 27

Gram + Cocci; catalase -; Beta hemolytic; Bacitracin Sensitive

Answer 27

Group A strep - S. pyogenes

Question 28

Gram+ cocci; catalase -; beta hemolytic; bacitracin resistant

Answer 28

Group B strep - S. agalactiae

Question 29

Gram + cocci; catalase -; gamma hemolytic - growth in bile and 6.5% NaCl

Answer 29

E. faecalis. Group D strep (enterococcus)

Question 30

Gram + Cocci; Catalse -; gamma hemolytic - growth in bile NOT in 6.5% NaCl

Answer 30

Non-enterococcus - S. bovis

Question 31

Gram -; Oxidase +; comma shaped; Grows in 42 C

Answer 31

Campylobacter jejuni

Question 32

Gram -; oxidase +; comma shaped; grows in alkaline media

Answer 32

Vibrio Cholerae

Question 33

Gram -; Diplococci; Maltose fermenter

Answer 33

N. meningitidis

Question 34

Gram -; Diplococci; Maltose non-fermenter

Answer 34

N. gonorrhoeae

Question 35

Gram -; rod; lactose fermenter (fast) - [3]

Answer 35

Klebsiella; E. coli; Enterobacter

Question 36

Gram - rod; slow lactose fermenter [2]

Answer 36

Citrobacter; Serratia

Question 37

Gram - rod; lactose non-fermenter; Oxidase - [3]

Answer 37

Shigella; Salmonella; Proteus

Question 38

Gram - rod; lactose non-fermenter; oxidase + [2]

Answer 38

Pseudomonas; H. pylori

Question 39

E. coli strain that does NOT ferment sorbitol

Answer 39

EHEC O157:H7. all others do