Micro Lecture Exam 2 Flashcards

Metabolism in Bacteria,

1
Q

Metabolism

A

All of the chemical reactions that occur in a cell (making and using energy)

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2
Q

What are the two types of metabolism?

A

Catabolism, anabolism

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3
Q

Catabolism

A

chemical reactions that releases energy (ATP & reduced coenzymes) to BREAK DOWN large molecules into smaller ones

Catabolic reactions: Krebs cycle, glycolysis, fermentation

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4
Q

Anabolism (biosynthesis)

A

chemical reactions that use energy to BUILD larger cell structures

Anabolic reactions: Polypeptide synthesis & cell assembly

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5
Q

Enzymes

A

protein catalysts that speed up chemical reactions by lowering the energy of activation

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6
Q

Energy of activation

A

an energy barrier that represents the minimum amount of energy needed in order for a chemical reaction to occur

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7
Q

Purpose of enzymes

A

Provide a site for specific reactions to happen

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8
Q

Substrates

A

The molecules that attach to the active site of an enzyme molecule

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9
Q

Whats the common suffix for enzymes?

A

-ase

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10
Q

Holoenzyme

A

An enzyme structure that can consist both protein and non protein molecules

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11
Q

Process of chemical reaction with enzymes

A

each new substrate is the product of the previous reaction which leads to the formation of a new product

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12
Q

Simple enzyme

A

Consists of proteins only

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13
Q

Apoenzyme

A

the protein part of an enzyme

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14
Q

What is the non-protein portion called?

A

Cofactors

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15
Q

Two types of cofactors

A

Metallic (iron, copper, magnesium)
Coenzymes (vitamins)

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16
Q

Catalase

A

An enzyme with an iron cofactor that breaks down hydrogen peroxide

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17
Q

What are the structures of an enzyme?

A

Primary, secondary, tertiary, and some quaternary

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18
Q

Active Site

A

Where substrate binds to enzyme and represent a “Lock & Key” rule

19
Q

What happens when an appropriate reaction occurs?

A

It develops and is released by the enzyme

20
Q

What factors of an organism’s habitat do enzymes operate under?

A

Temperature, radiation, electricity, pH, and the osmotic pressure

21
Q

What happens if enzymes are made to change because of the organisms habitat?

A

They become unstable and metabolic function fails which leads to cell death

22
Q

Denaturation

A

When the shape of the enzyme is no longer maintained and the bonds break making the enzyme no longer functional

23
Q

Exoenzymes

A

They leave the cells to break down large food molecules or harmful chemicals

24
Q

Endoenzymes

A

Stay inside the cell

25
Q

Are majority enzymes endoenzymes or exoenzymes?

A

Endoenzymes

26
Q

Virulence Factors

A

molecules produced by pathogens that help the pathogens to invade host tissues, evade the host immune response, and cause damage to host cells or tissues

27
Q

Why do pathogens use exoenzymes?

A

To help them evade host defenses

28
Q

Forms of energy

A

thermal, radiant, electrical, mechanical, atomic, and chemical

29
Q

What chemical energy is used?

A

Energy transfers from ADP to ATP which stores energy to be released

30
Q

What are the 3 catabolic pathways that convert glucose to CO2 and give off energy?

A

Glycolysis,Kreb’s, and ETC

31
Q

Krebs Cycle

A

a series of enzyme catalyzed reactions

32
Q

Glycolysis

A

series of oxidation reactions which means electrons are being removed; splitting sugar

33
Q

Pathway of aerobic respiration

A

glycolysis, transition, Kreb’s cycle, electron transport chain; oxygen is the final electron acceptor

34
Q

Pathway of anaerobic respiration

A

glycolysis, transition, Kreb’s cycle, electron transport chain; oxygen is not the final electron acceptor

35
Q

Pathway of Fermentation

A

Glycolysis only; organic compounds are the final electron acceptors

36
Q

Why is fermentation important?

A

The microbes can grow just as fast without producting ATP by increasing the rate of glycolysis; leads to production of beer wine bread

37
Q

Which pathway represents burning fat at the gym for energy?

A

Catabolic pathway

38
Q

What are components of the bacterial cell?

A

Carbs, proteins, lipids

39
Q

6CO2 + 6H2O —> C6H12O6 + 6O2

A

trap sunlight and use it to produce high-energy glucose from low energy CO2 and water.

40
Q

Aerobic Respiration

A

transfer of electrons from glucose to oxygen via ETC

41
Q

Reduced coenzyme (NADH & FADH)

A

found in all living cells and represent electron carriers for ETC

42
Q

Electron Transport Chain

A

series of proteins and carriers embedded in the cell membrane and uses electron flow to pass the electrons from one molecule to the next

43
Q

Fermentation

A

the incomplete breakdown of glucose due the absence of an electron acceptor for the electron transport system

44
Q

Lipases

A

enzymes that breaks down fatty acids a glycerol