Micro. Lab Mid Term Flashcards

1
Q

What is the Scientific Method

A

OBSERVATION, QUESTIONS about observation, form HYPOTHESIS, conduct EXPERIMENTS, draw logical CONCLUSION. REPEAT as necessary.

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2
Q

REDUCING MEDIA

A

removes O2

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3
Q

ENRICHED MEDIA

A

proved extra nutrients

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4
Q

SELECTIVE MEDIA

A

prevents growth of unwanted organisms

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5
Q

DIFFERENTIAL MEDIA

A

allows discrimination of organisms

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6
Q

CAPNOPHILES

A

extra CO2

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7
Q

AUTOCLAVEING

A

kills endospores. Temp of 121* for 15 minutes at 15 PSI

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8
Q

PURE CULTURE

A

only one kind of microorganism

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9
Q

MIXED CULTURE

A

more than one kind of microorganism

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10
Q

How to measure dead cells

A

Visual Examination
Electronic Cell Counters
Turbidity
Dry WEight

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11
Q

STERILIZATION

A

Removal of ALL microorganism including endospores

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12
Q

DISINFECTION

A

removal off non endospore forming pathogens on inanimate (nonliving) objects.

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13
Q

ANTISEPTIC

A

Removal of non endospore forming pathogens on LIVING things.

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14
Q

DEGERMING

A

removal of microorganisms from a limited area.

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15
Q

SANITIZING

A

treatment that lowers the number of microorganisms to a safe level for the public

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16
Q

BACTERICIDAL

A

kills bacteria

17
Q

BACTERIOSTATIC

A

inhibits bacterial growth without killing bacteria

18
Q

MEDICAL ASEPSIS (clean technique)

A

Sanitization:
degerm hands
disinfection
PPE

19
Q

SURGICAL ASEPSIS (sterile technique)

A
STERILIZATION:
intensive degerming
intensive disinfection
sterile PPE
Sterile equipment
20
Q

PARFOCAL

A

the image remains nearly infocus.

21
Q

CONTRAST

A

difference in brightness and color. We want dark contrast.

22
Q

HELMINTHS

A

worms

23
Q

PROTOZOA

A

mobile, moist environment

24
Q

FUNGI

A

yeast and molds