Micro. Lab Mid Term Flashcards
What is the Scientific Method
OBSERVATION, QUESTIONS about observation, form HYPOTHESIS, conduct EXPERIMENTS, draw logical CONCLUSION. REPEAT as necessary.
REDUCING MEDIA
removes O2
ENRICHED MEDIA
proved extra nutrients
SELECTIVE MEDIA
prevents growth of unwanted organisms
DIFFERENTIAL MEDIA
allows discrimination of organisms
CAPNOPHILES
extra CO2
AUTOCLAVEING
kills endospores. Temp of 121* for 15 minutes at 15 PSI
PURE CULTURE
only one kind of microorganism
MIXED CULTURE
more than one kind of microorganism
How to measure dead cells
Visual Examination
Electronic Cell Counters
Turbidity
Dry WEight
STERILIZATION
Removal of ALL microorganism including endospores
DISINFECTION
removal off non endospore forming pathogens on inanimate (nonliving) objects.
ANTISEPTIC
Removal of non endospore forming pathogens on LIVING things.
DEGERMING
removal of microorganisms from a limited area.
SANITIZING
treatment that lowers the number of microorganisms to a safe level for the public
BACTERICIDAL
kills bacteria
BACTERIOSTATIC
inhibits bacterial growth without killing bacteria
MEDICAL ASEPSIS (clean technique)
Sanitization:
degerm hands
disinfection
PPE
SURGICAL ASEPSIS (sterile technique)
STERILIZATION: intensive degerming intensive disinfection sterile PPE Sterile equipment
PARFOCAL
the image remains nearly infocus.
CONTRAST
difference in brightness and color. We want dark contrast.
HELMINTHS
worms
PROTOZOA
mobile, moist environment
FUNGI
yeast and molds