Micro Lab findings Flashcards

1
Q

Beta-hemolytic bacteria

A

S. aureus, S. pyogenes (Group B), S. agalactiae (Group A) and Listeria

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2
Q

Alpha hemolytic bacteria

A

Enterococci, S. pneumo Viridans strep

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3
Q

Gamma hemolytic bacteria

A

Enterococci

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4
Q

Bile-esculin agar

A

Enterococci

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5
Q

CAMP positive bacteria

A

S. agalactiae, Listeria

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6
Q

Bordet-Gengou agar

A

Bordatella pertussis

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7
Q

BCYE agar

A

Legionella

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8
Q

Chocolate agar

A

H. influenza, N. meningitidis

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9
Q

Lowenstein-Jensen agar

A

Mycobacterium tuberculosis

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10
Q

MacConkey Agar

A

Klebsiella, E. coli (lactose fermenters)

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11
Q

What is positive when looking at MacConkey Agar

A

Pink (positive pink); means the bacteria ferments lactose

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12
Q

Regan-Lowe agar

A

Bordetalla pertussis (has replaced Bordet-Gengou as agar of choice for this)

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13
Q

Sabouraud dextrose agar

A

Fungi

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14
Q

Sorbitol-MacConkey

A

EHEC (does not ferment sorbitol like other E. coli species)

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15
Q

Thayer-Martin agar

A

Neisseria

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16
Q

Thiosulfate citrate bile salts sucrose agar (TCBS)

A

Vibrio spp.

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17
Q

Eaton medium

A

Mycoplasma pneumonia

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18
Q

Egg yolk agar

A

Clostridium perfringens

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19
Q

Eosin Methylene Blue agar (EMB)

A

E. coli; grows as colonies with a green metallic sheen

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20
Q

Hektoen enteric agar

A

Salmonella and Shigella

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21
Q

Loeffler medium

A

Corynebacterium diptheriae

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22
Q

Mannitol salt agar

A

S. aureus (turns agar yellow)

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23
Q

Tellurite agar

A

C. diptheriae

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24
Q

Xylose-Lysine-Deoxycholate (XLD) agar

A

Salmonella and shigella

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25
Q

The only gram (-) cocci

A

Neisseria spp.

26
Q

Gram + rods

A

Clostridium, corynebacterium, Bacillus, Listeria, Mycobacterium, Gardnerella

27
Q

Bacteria that have mycolic acid wall with high lipid content

A

Mycobacterium

28
Q

Bacteria that contain sterols in the membrane but have no cell wall

A

Mycoplasma

29
Q

Giemsa stain

A

Certain Bugs Really Try my Patience;

Chlamydia, Borrelia, Rickettsiae, Trypanosomes, Plasmodium

30
Q

Periodic acid-Schiff stain (PAS)

A

Trophyrema whipplei

31
Q

India ink

A

Cryptococcus (mucicarmine stain that stains polysaccharide capsule RED)

32
Q

Silver stain

A

Fungi, Legionella, Helicobacter pylori

33
Q

Obligate aerobes

A

Nagging Pests Must Breathe;

Nocardia, Pseudomonas, Mycobacterium TB, Bacillus

34
Q

Obligate anaerobes

A

Can’t Breathe Air;

Clostridium, Bacteroides, Actinomyces (A. israeli is a facultative anaerobe)

35
Q

Obligate intracellular

A

Ricketsia, Chlamydia

36
Q

Facultative intracellular

A

Some Nasty Bugs May Live FacultativeLY;

Salmonella, Neisseria, Brucella, Mycobacterium, Listeria, Francisella, Legionella, Yersinia pestis

37
Q

Encapsulated bacteria

A

SHiNE SKiS;

S. pneumo, H. influenzae type B, N. meningitidis, E. coli, Salmonella, Klebsiella, S. pyogenes (group B Strep)

38
Q

Catalase + organisms

A

PLACESS;

Pseudomonas, Listeria, aspergillus, Candida, E. coli, S. aureus, Serratia

39
Q

Coagulase + organism

A

S. aureus

40
Q

Catalase + coagulase negative organisms

A

S. saprophyticus, s. epidermidis

41
Q

Novobiocin sensitive organism

A

S. epidermidis

42
Q

Novobiocin resistant organism

A

S. saprophyticus

43
Q

Bile-esculin +

A

Enterococcus

44
Q

Bile-esculin -

A

Viridans strep, strep pneumoniae

45
Q

Optochin sensitive organism

A

S. pneumo

46
Q

Optochin resistance

A

Viridans strep

47
Q

Bacitracin sensitive

A

S. pyogenes

48
Q

Bacitracin resistant

A

S. agalactiae

49
Q

Doesn’t ferment glucose, oxidase +

A

Pseudomonas

50
Q

Glucose fermenters, oxidase -

A

Klebsiella, E. coli, Salmonella, Shigella, Proteus

KESSP

51
Q

Lactose fermenters

A

Kleb, E. coli

52
Q

Lactose non-fermenters

A

Salmonella, Shigella, Proteus

53
Q

H2S -

A

Shigella

54
Q

H2S +

A

Salmonella, Proteus

55
Q

Indole +

A

E. coli

56
Q

Indole -

A

Klebsiella

57
Q

Urease +

A

Proteus

58
Q

Urease -

A

Salmonella

59
Q

Turns red on mucicarmine stain

A

Cryptococcus

60
Q

E. coli that DOESN’T ferment sorbitol

A

EHEC

61
Q

Flask-shaped ulcer

A

Entamoeba histolytica