Micro Lab findings Flashcards
Beta-hemolytic bacteria
S. aureus, S. pyogenes (Group B), S. agalactiae (Group A) and Listeria
Alpha hemolytic bacteria
Enterococci, S. pneumo Viridans strep
Gamma hemolytic bacteria
Enterococci
Bile-esculin agar
Enterococci
CAMP positive bacteria
S. agalactiae, Listeria
Bordet-Gengou agar
Bordatella pertussis
BCYE agar
Legionella
Chocolate agar
H. influenza, N. meningitidis
Lowenstein-Jensen agar
Mycobacterium tuberculosis
MacConkey Agar
Klebsiella, E. coli (lactose fermenters)
What is positive when looking at MacConkey Agar
Pink (positive pink); means the bacteria ferments lactose
Regan-Lowe agar
Bordetalla pertussis (has replaced Bordet-Gengou as agar of choice for this)
Sabouraud dextrose agar
Fungi
Sorbitol-MacConkey
EHEC (does not ferment sorbitol like other E. coli species)
Thayer-Martin agar
Neisseria
Thiosulfate citrate bile salts sucrose agar (TCBS)
Vibrio spp.
Eaton medium
Mycoplasma pneumonia
Egg yolk agar
Clostridium perfringens
Eosin Methylene Blue agar (EMB)
E. coli; grows as colonies with a green metallic sheen
Hektoen enteric agar
Salmonella and Shigella
Loeffler medium
Corynebacterium diptheriae
Mannitol salt agar
S. aureus (turns agar yellow)
Tellurite agar
C. diptheriae
Xylose-Lysine-Deoxycholate (XLD) agar
Salmonella and shigella
The only gram (-) cocci
Neisseria spp.
Gram + rods
Clostridium, corynebacterium, Bacillus, Listeria, Mycobacterium, Gardnerella
Bacteria that have mycolic acid wall with high lipid content
Mycobacterium
Bacteria that contain sterols in the membrane but have no cell wall
Mycoplasma
Giemsa stain
Certain Bugs Really Try my Patience;
Chlamydia, Borrelia, Rickettsiae, Trypanosomes, Plasmodium
Periodic acid-Schiff stain (PAS)
Trophyrema whipplei
India ink
Cryptococcus (mucicarmine stain that stains polysaccharide capsule RED)
Silver stain
Fungi, Legionella, Helicobacter pylori
Obligate aerobes
Nagging Pests Must Breathe;
Nocardia, Pseudomonas, Mycobacterium TB, Bacillus
Obligate anaerobes
Can’t Breathe Air;
Clostridium, Bacteroides, Actinomyces (A. israeli is a facultative anaerobe)
Obligate intracellular
Ricketsia, Chlamydia
Facultative intracellular
Some Nasty Bugs May Live FacultativeLY;
Salmonella, Neisseria, Brucella, Mycobacterium, Listeria, Francisella, Legionella, Yersinia pestis
Encapsulated bacteria
SHiNE SKiS;
S. pneumo, H. influenzae type B, N. meningitidis, E. coli, Salmonella, Klebsiella, S. pyogenes (group B Strep)
Catalase + organisms
PLACESS;
Pseudomonas, Listeria, aspergillus, Candida, E. coli, S. aureus, Serratia
Coagulase + organism
S. aureus
Catalase + coagulase negative organisms
S. saprophyticus, s. epidermidis
Novobiocin sensitive organism
S. epidermidis
Novobiocin resistant organism
S. saprophyticus
Bile-esculin +
Enterococcus
Bile-esculin -
Viridans strep, strep pneumoniae
Optochin sensitive organism
S. pneumo
Optochin resistance
Viridans strep
Bacitracin sensitive
S. pyogenes
Bacitracin resistant
S. agalactiae
Doesn’t ferment glucose, oxidase +
Pseudomonas
Glucose fermenters, oxidase -
Klebsiella, E. coli, Salmonella, Shigella, Proteus
KESSP
Lactose fermenters
Kleb, E. coli
Lactose non-fermenters
Salmonella, Shigella, Proteus
H2S -
Shigella
H2S +
Salmonella, Proteus
Indole +
E. coli
Indole -
Klebsiella
Urease +
Proteus
Urease -
Salmonella
Turns red on mucicarmine stain
Cryptococcus
E. coli that DOESN’T ferment sorbitol
EHEC
Flask-shaped ulcer
Entamoeba histolytica