Micro Lab findings

Question 1

Beta-hemolytic bacteria

Answer 1

S. aureus, S. pyogenes (Group B), S. agalactiae (Group A) and Listeria

Question 2

Alpha hemolytic bacteria

Answer 2

Enterococci, S. pneumo Viridans strep

Question 3

Gamma hemolytic bacteria

Answer 3

Enterococci

Question 4

Bile-esculin agar

Answer 4

Enterococci

Question 5

CAMP positive bacteria

Answer 5

S. agalactiae, Listeria

Question 6

Bordet-Gengou agar

Answer 6

Bordatella pertussis

Question 7

BCYE agar

Answer 7

Legionella

Question 8

Chocolate agar

Answer 8

H. influenza, N. meningitidis

Question 9

Lowenstein-Jensen agar

Answer 9

Mycobacterium tuberculosis

Question 10

MacConkey Agar

Answer 10

Klebsiella, E. coli (lactose fermenters)

Question 11

What is positive when looking at MacConkey Agar

Answer 11

Pink (positive pink); means the bacteria ferments lactose

Question 12

Regan-Lowe agar

Answer 12

Bordetalla pertussis (has replaced Bordet-Gengou as agar of choice for this)

Question 13

Sabouraud dextrose agar

Answer 13

Fungi

Question 14

Sorbitol-MacConkey

Answer 14

EHEC (does not ferment sorbitol like other E. coli species)

Question 15

Thayer-Martin agar

Answer 15

Neisseria

Question 16

Thiosulfate citrate bile salts sucrose agar (TCBS)

Answer 16

Vibrio spp.

Question 17

Eaton medium

Answer 17

Mycoplasma pneumonia

Question 18

Egg yolk agar

Answer 18

Clostridium perfringens

Question 19

Eosin Methylene Blue agar (EMB)

Answer 19

E. coli; grows as colonies with a green metallic sheen

Question 20

Hektoen enteric agar

Answer 20

Salmonella and Shigella

Question 21

Loeffler medium

Answer 21

Corynebacterium diptheriae

Question 22

Mannitol salt agar

Answer 22

S. aureus (turns agar yellow)

Question 23

Tellurite agar

Answer 23

C. diptheriae

Question 24

Xylose-Lysine-Deoxycholate (XLD) agar

Answer 24

Salmonella and shigella

Question 25

The only gram (-) cocci

Answer 25

Neisseria spp.

Question 26

Gram + rods

Answer 26

Clostridium, corynebacterium, Bacillus, Listeria, Mycobacterium, Gardnerella

Question 27

Bacteria that have mycolic acid wall with high lipid content

Answer 27

Mycobacterium

Question 28

Bacteria that contain sterols in the membrane but have no cell wall

Answer 28

Mycoplasma

Question 29

Giemsa stain

Answer 29

Certain Bugs Really Try my Patience; Chlamydia, Borrelia, Rickettsiae, Trypanosomes, Plasmodium

Question 30

Periodic acid-Schiff stain (PAS)

Answer 30

Trophyrema whipplei

Question 31

India ink

Answer 31

Cryptococcus (mucicarmine stain that stains polysaccharide capsule RED)

Question 32

Silver stain

Answer 32

Fungi, Legionella, Helicobacter pylori

Question 33

Obligate aerobes

Answer 33

Nagging Pests Must Breathe; Nocardia, Pseudomonas, Mycobacterium TB, Bacillus

Question 34

Obligate anaerobes

Answer 34

Can't Breathe Air; Clostridium, Bacteroides, Actinomyces (A. israeli is a facultative anaerobe)

Question 35

Obligate intracellular

Answer 35

Ricketsia, Chlamydia

Question 36

Facultative intracellular

Answer 36

Some Nasty Bugs May Live FacultativeLY; Salmonella, Neisseria, Brucella, Mycobacterium, Listeria, Francisella, Legionella, Yersinia pestis

Question 37

Encapsulated bacteria

Answer 37

SHiNE SKiS; S. pneumo, H. influenzae type B, N. meningitidis, E. coli, Salmonella, Klebsiella, S. pyogenes (group B Strep)

Question 38

Catalase + organisms

Answer 38

PLACESS; Pseudomonas, Listeria, aspergillus, Candida, E. coli, S. aureus, Serratia

Question 39

Coagulase + organism

Answer 39

S. aureus

Question 40

Catalase + coagulase negative organisms

Answer 40

S. saprophyticus, s. epidermidis

Question 41

Novobiocin sensitive organism

Answer 41

S. epidermidis

Question 42

Novobiocin resistant organism

Answer 42

S. saprophyticus

Question 43

Bile-esculin +

Answer 43

Enterococcus

Question 44

Bile-esculin -

Answer 44

Viridans strep, strep pneumoniae

Question 45

Optochin sensitive organism

Answer 45

S. pneumo

Question 46

Optochin resistance

Answer 46

Viridans strep

Question 47

Bacitracin sensitive

Answer 47

S. pyogenes

Question 48

Bacitracin resistant

Answer 48

S. agalactiae

Question 49

Doesn't ferment glucose, oxidase +

Answer 49

Pseudomonas

Question 50

Glucose fermenters, oxidase -

Answer 50

Klebsiella, E. coli, Salmonella, Shigella, Proteus KESSP

Question 51

Lactose fermenters

Answer 51

Kleb, E. coli

Question 52

Lactose non-fermenters

Answer 52

Salmonella, Shigella, Proteus

Question 53

H2S -

Answer 53

Shigella

Question 54

H2S +

Answer 54

Salmonella, Proteus

Question 55

Indole +

Answer 55

E. coli

Question 56

Indole -

Answer 56

Klebsiella

Question 57

Urease +

Answer 57

Proteus

Question 58

Urease -

Answer 58

Salmonella

Question 59

Turns red on mucicarmine stain

Answer 59

Cryptococcus

Question 60

E. coli that DOESN'T ferment sorbitol

Answer 60

EHEC

Question 61

Flask-shaped ulcer

Answer 61

Entamoeba histolytica