Micro Intro Lab

Question 1

Sample is placed in a
container of growth
medium

Answer 1

INOCULATION

Question 2

Creates the proper
growth with respect to
temperature and gas
requirements

Answer 2

INCUBATION

Question 3

Once the cultures have grown, they may
need to be reinoculated (and incubated) in such a way that separate species are obtained

Answer 3

Isolation

Question 4

Samples are observed macroscopically and
microscopically, possibly with the aid of staining

Answer 4

Inspection

Question 5

The identity of the isolated microbe is determined, usually to the species level. Inspection may not be
enough to identify some microbes, but additional techniques include biochemical tests, immunologic
tests, and genetic analysis

Answer 5

Identification

Question 6

3 ways to inoculate

Answer 6

1. Streak plate method
2. Pour plate method
3. Spread plate method

Question 7

Uses ultraviolet radiation as the illuminating
source

Answer 7

Fluorescence microscope

Question 8

Uses a laser beam as the illuminating source

Answer 8

Confocal microscope