micro final Flashcards

1
Q

how to ddx small cell lymphoma from normal lymphocytes

A

flow cytometry PARR

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2
Q

what is the normal size of a small lymphocyte

A

1-1.5x a red cell

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3
Q

when suspecting lymphoma what is best to run

A

best to run both flow and parr

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4
Q

what is used for phenotyping of lymphoid neoplasia

A

flow

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5
Q

acute diarrhea in a watery young dog

A

protozoa. likely giardia

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6
Q

what is the size of giardia

A

12 ish micrometers

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7
Q

what does a fecal float detect for giardia

A

cysts

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8
Q

what test detects trophozoites of giardia

A

direct smear. they die fast. fresh feces is a must

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9
Q

when to test a dog for giardia

A

symptomatic dogs with diarrhea

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10
Q

treatment for giardia

A

fenbendazole. or metronidazole

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11
Q

do we need a definitive diagnosis for salmonella

A

yes. zoonotic and may chronically shed

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12
Q

what signs support salmonella in a horse

A

neutropenia, l shift, bands, fever

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13
Q

what is the test of choice for salmonella in a horse

A

salmonella PCR and culture

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14
Q

do you need antimicrobial susceptibility testing for salmonella positive

A

no. controversial

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15
Q

what is the treatment for enteric salmonellosis

A

does not change the course of colitis. will prolong shedding of the bacteria.

dont give it. give polymixin B and banamine

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16
Q

how is equine salmonellosis intermittently shedding

A

persists intracellularly in enterocytes and macrophages

17
Q

how do you clear a salmonella positive animal

A

5 negative cultures or isolation for 30 days after cessation of clinical signs

18
Q

how do we diagnose johnes disease

A

pcr and serology

19
Q

what test would you choose for a fecal sample for johnes disease

20
Q

if we saw a johnes positive fecal PCR but a serum elisa negative. how could that be?

A

the elisa has poor sensitivity. trust the PCR

21
Q

what is unique about the johnes serology

A

there is a flux in antibodies so the timing has to be perfect.

22
Q

how is johnes transmitted

A

fecal oral to babies. separate offspring at birth

23
Q

what type of infection does johnes disease cause

A

granulomatous inflammation. malabsorptive diarrhea, poor bcs, and hypoproteinemia

24
Q

can cattle get sheep johnes?

A

yes and visa versa

25
the most cost effective tool we have for herd level serveillance of johnes?
serology (elisa). trust the positives not the negatives
26
how does feline coronavirus turn into FIP
mutation so that it now replicated in macrophages instead of enterocytes
27
what percentage of catteries are seropositive for coronavirus
80%
28
what is FIP
fatal multisystemic granulomatous disease of young cats
29
what is one of the reasons FIP kills
causes increase in antibody/antigen complexes that deposit in the vessels causing vasculitis
30
gold standard (post mortem) FIP diagnosis
histo/IHC
31
what sample type for PCR detection of FIP is the most helpful
PCR of abdominal fluid
32
what does the rivalta abdominal fluid test do
distinguishes between protein rich fluid or transudate.. + is jellyfish
33
what is a good differential for FIP and what test would you run
histoplasma. run a urine antigen test
34
what is the gold standard test for lepto
MAT- detects IgM
35
what lepto test is at high risk for false negatives
fluorescent antibody test - FA
36
what to remember about the lepto snap test
can potentially cross react with vaccine. but if you are exposed you should be sick
37
what are lepto MAT results
titers. if doing paired tests look for a 4 fold increase
38
what acute MAT titer results would tell us of an infection
>1:800