Michaelis Menten Plot Flashcards
Michaelis menten plot equation
how should a michaelis menten graph plot?
as a curve
what does the V stand out for in the Michaelis menten equation?
rate of enzyme catalysed reaction
what does vmax stand out for in the michaelis menten equation?
theoretical maximum reaction rate attainable.
what does kM stand out for in the Michaelis menten equation?
Michaelis constant: the substrate concentration at 50% of maximum reaction rate.
how can michaelis menten plots be generated?
a series of enzyme kinetics reactions.
what is vmax on Michaelis menten graph? and how is KM found by this?
rate of reaction where the MM plot will theoretically plateau.
KM is found by reading the substrate concentration of the graph at vmax/2 (from y axis)
What are Michaelis menten plots good for?
first impressions of how reaction rates varies with substrate concentration.
why are linweaver birk plots used?
used to display enzyme kinetics data.
how are linweaver birk plots different to michealies menter plots?
they are double reciprocal transforms of michaelis menten plots and can be used to more accurately estimate key values of Km and Vmax.
linweaver-berk equation