Michaelis Menten Plot Flashcards

1
Q

Michaelis menten plot equation

A
How well did you know this?
1
Not at all
2
3
4
5
Perfectly
2
Q

how should a michaelis menten graph plot?

A

as a curve

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
3
Q

what does the V stand out for in the Michaelis menten equation?

A

rate of enzyme catalysed reaction

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
4
Q

what does vmax stand out for in the michaelis menten equation?

A

theoretical maximum reaction rate attainable.

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
5
Q

what does kM stand out for in the Michaelis menten equation?

A

Michaelis constant: the substrate concentration at 50% of maximum reaction rate.

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
6
Q

how can michaelis menten plots be generated?

A

a series of enzyme kinetics reactions.

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
7
Q

what is vmax on Michaelis menten graph? and how is KM found by this?

A

rate of reaction where the MM plot will theoretically plateau.
KM is found by reading the substrate concentration of the graph at vmax/2 (from y axis)

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
8
Q

What are Michaelis menten plots good for?

A

first impressions of how reaction rates varies with substrate concentration.

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
9
Q

why are linweaver birk plots used?

A

used to display enzyme kinetics data.

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
10
Q

how are linweaver birk plots different to michealies menter plots?

A

they are double reciprocal transforms of michaelis menten plots and can be used to more accurately estimate key values of Km and Vmax.

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
11
Q

linweaver-berk equation

A
How well did you know this?
1
Not at all
2
3
4
5
Perfectly