michaelis menten kinetics specific

Question 1

v

Answer 1

reaction velocity- rate of P formation

Question 2

Vmax

Answer 2

ax velocity of which enzyme catalyse the reaction

Question 3

Km

Answer 3

michaelis constant - [s] at 1/2 Vmax

Question 4

system will not reach Vmax until

Answer 4

[s] is much bigger than Km

Question 5

michaelis menten equation

Answer 5

V= Vmax x [s]
—————–
Km + [s]

Question 6

what is Vmax equal to

Answer 6

Kcat

Question 7

Kcat

Answer 7

catalyst rate constant

Question 8

if we add more substrate rate of formation of P will

Answer 8

increase–> most enzymes follow this pattern and eventually Vmax will be reached

Question 9

what does it mean for enzymes when Vmax is reached

Answer 9

all active sites are full

Question 10

how can we increase Vmax

Answer 10

by adding more enzymes

Question 11

Vmax is determined by

Answer 11

the amount of enzymes present

Question 12

Km has the same units as

Answer 12

[s]

Question 13

at some point Km must be equal to

Answer 13

[s]

Question 14

km=

Answer 14

VMAX/2

Question 15

if small Km

Answer 15

VMAX is reached at low [s]

Question 16

if large Km

Answer 16

VMAX is reached at high [s]

Question 17

when Km is very small it can be

Answer 17

cancelled out of the equation all together, therefore so can the [s] on both sides and therefore V=VMAX

Question 18

when V=VMAX

Answer 18

system is running at Vmax all the time

Question 19

what is the M-M equation converts to when it comes to line weaver burk plot

Answer 19

an equation for a straight line

Question 20

at the y intercept

Answer 20

1/VMAX

Question 21

at the x intercept

Answer 21

-1/Km

Question 22

straight line equation

Answer 22

y=mx+ c

Question 23

hanes woolf..

Answer 23

provides more accuracy

Question 24

on hanes woolf gradient=

Answer 24

1/vmax

Question 25

on hanes woolf plot y intercept =

Answer 25

km/vmax