michaelis menten kinetics specific Flashcards
1
Q
v
A
reaction velocity- rate of P formation
2
Q
Vmax
A
ax velocity of which enzyme catalyse the reaction
3
Q
Km
A
michaelis constant - [s] at 1/2 Vmax
4
Q
system will not reach Vmax until
A
[s] is much bigger than Km
5
Q
michaelis menten equation
A
V= Vmax x [s]
—————–
Km + [s]
6
Q
what is Vmax equal to
A
Kcat
7
Q
Kcat
A
catalyst rate constant
8
Q
if we add more substrate rate of formation of P will
A
increase–> most enzymes follow this pattern and eventually Vmax will be reached
9
Q
what does it mean for enzymes when Vmax is reached
A
all active sites are full
10
Q
how can we increase Vmax
A
by adding more enzymes
11
Q
Vmax is determined by
A
the amount of enzymes present
12
Q
Km has the same units as
A
[s]
13
Q
at some point Km must be equal to
A
[s]
14
Q
km=
A
VMAX/2
15
Q
if small Km
A
VMAX is reached at low [s]
