Mice Flashcards
What is the scientific name for mice?
Mus musculus
What are the 3 main categories of mice?
Outbred stocks, inbred strains and F1 hybrids
What are the 4 ecologic type of mice?
Axenic (free of microbes not needed to sustain life), Gnotobiotic (known microflora), Specific pathogen free, conventional (animals with undefined microflora)
How many generations does it take to make inbred strains?
20 or more
How much homozygosity do inbred strains have?
greater than 97%
What do athymic nude mice lack?
T cells
SCID mice lack what?
Both T and B cells
What are F1 hybrids?
The first progeny between 2 different inbred strains
How are transgenic mice made?
When foreign DNA is introduced into the cells during early embryonic development
How are knockout mice made?
Introducing a transgene, knocks out specific genes to study loss of function
What is it called when a mouse takes the whiskers off another mouse?
Barbering
Which teeth are hypsodont and which are brachydont?
Incisors are hypsodont, molars are brachydont
What kind of fat do mice use to keep warm?
Brown fat
What is the lifespan of a mouse?
1.5-3 years
What is the mouse gestation period?
19-21 days
What is the weaning period?
21-28 days
What is the mouse litter size?
10-12
What is the Whitten effect?
Females introduced to males will cycle within 3 days
What is the Bruce effect?
If a pregnant female smells a strange male within 4 days of conception, she will abort the current pregnancy
How much food do mice consume?
3-5 g per day
What must you do to a mouse before getting blood?
Anesthetize it
Which veins are good for a small amount of blood in rats?
Dorsal tail artery or clipped toenail
Which vein is good for a larger volume of blood?
Jugular vein
What is the max amount of blood that can be taken in mice? How much blood do mice have in their bodies?
0.2 ml is the max.
Mice have 1.6-3.2 ml of blood
What is the max amount that can be injected into a mouse?
1-2 ml
What are the needle sizes used to give injections?
23 and 25 gauge
What pain medications is recommended postop for rodents?
Butorphenol
How much saline can be given to mice during anesthesia?
1-2 ml per 100 g body weight
What is the preferred method of euthanasia for rodents?
CO2 chamber
What is a good antibiotic to treat pneumonia?
Enrofloxacin (Baytril)
What does Helicobacter cause in the body? What is it associated with?
Active chronic hepatitis
Associated with IBD.
What is the most common respiratory disease in mice?
Mycoplasma pulmonis
Tyzzer’s disease is caused by what?
Clostridium pilaforme
What does Citrobacter freundii cause? How is it transmitted?
Transmissible murine colonic hyperplasia
Transmitted via fecal/oral route
What are clinical signs of Transmissible murine colonic hyperplasia?
Depression, anorexia, hunched posture, dehydration, rough hair coat, rectal prolapse
What are clinical signs of Staph?
Dermatitis, foot swelling, SQ lumps, skin lesions, pneumonia, toxemia, septicemia
How can pseudomonas aeruginosa be avoided?
By chlorinating drinking water to a pH of 2.5-2.8
Strep is associated with what?
Dermatitis
Corynebacterium pseudodiptheriticum is associated with what?
Severe hyperkeratotic dermatitis in nude mice, flaky skin and pruritis
Which zoonotic bacteria can come from mice?
Salmonella
What is the most common viral infection in mice?
Sendai virus
How do you burn out Mouse Hepatitis Virus?
Cease breeding for 3-6 weeks
What is a zoonotic virus that can come from mice and is of public health importance?
Lymphocytic Choriomeningitis (LCM)
What is a rare pox virus that can come from mice?
Ectromelia
What is Myobia musculi and what is Polyplax serrata?
A mite and a louse
What is the best bedding for mice and rats?
Paper
What are knockout mice used for?
Immunological research
How are oral medications given?
Gavage
What type of reproductive cycle do mice have?
Polyestrus, but are NOT induced ovulators
What are transgenic mice used for?
Genetic biomedical research
What is the house mouse louse?
Polyplax serrata
