MHC Flashcards
What is component of MHC 1?
All nucleated cells
What is component of MHC 2?
APC - dendritic cell, b cell + macrophages
What is CD4+?
HELPER CELLS -> MHC 2
What is CD8+?
CYTOTOXIC -> MHC 1
how much alpha + beta in MHC 1?
3 alpha + 1 beta
how much alpha + beta in MHC 2?
2 alpha + 2 beta
how much Cytoplasmic tail in transmembrane region in MHC 1?
1
how much Cytoplasmic tail in transmembrane region in MHC 2?
2
Where is the peptide binding cleft/variable region in MHC 1?
between alpha 1 + 2
Where is the peptide binding cleft/ variable region in MHC 2?
between alpha + beta
What is cytoplasmic tail in transmembrane region?
for signal transduction
is alpha or beta needed for signal transduction in MHC 1?
alpha
Does MHC 1 go through membrane?
yes its transmembrane.
Which alpha in MHC 1 bind to CD8?
alpha 3
At the peptide binding cleft of MHC 1 - how many amino acids does it bind to?
8-10 amino acids
What beta is in MHC 1?
BETA 2 MICROGLOBULIN
Is beta necessary for MHC 1?
Without it we can’t make MHC 1
How does MHC 1 get expressed?
Virus enter –> phagosome –> ubiquinated –> peptide –> enter via TAP to ER –> MHC 1 go through er to golgi to vesicle –> MHC 1 go out.
What is HLA?
same as MHC
Where is binding cleft in MHC2?
alpha 1 + beta 1
Where is the conservative in MHC2?
alpha 2 + beta 2
At the peptide binding cleft of MHC 2 - how many amino acids does it bind to?
13-25 amino acids
Which beta in MHC 2 bind to CD4?
beta 2
How many locis encode for MHC 2?
5
How does MHC 2 get expressed?
antigen –> endolysosome –> ER –> MHC 2 + invarient chain –> golgi –> vesicle –> CLIP –> break invarient –> HLA –> break clip –> MHC 2
How many peptides bind at once in MHC?
1
What does MHC require of cells?
T
What increases level of MHC?
CYTOKINES
Peptides from ___ associate with MHC I
cytosol
Peptides from ___ associate with MHC 2
vesicles
HLAs in MHC 1:
HLA- a/b/c
HLAs in MHC 2:
HLA- D p/q/r
which MHC has endogenous antigens and which has exogenous antigens?
MHC 1: endogenous
2: exogenous
