Methods For Protein Seperation

Question 1

What is protein separation based on

Answer 1

Large diff in some physical and chemical characteristics

Question 2

What are the protein seperation techniques

Answer 2

Ultracentrifugation
Electrophoresis
Chromatography

Question 3

What is the definition of ultracentrifugation

Answer 3

Mechanical process involving the use of the centrifugal force to separate particles from a solution in high speed to purify macromolecules

Question 4

What is the heavy component called and what is the remaining liquid called

Answer 4

Precipitate

Supernatant

Question 5

Define electrophoresis

Answer 5

Separation based on differential of migration of charged particles in an applied electric field

Question 6

Whats the principal of electrophoresis

Answer 6

Sample is deposited on solid support which is soaked in a conducting solution
When charge is applied molecules dissolved move along at a rate corresponding to magnitude of their charge and mass

Question 7

What are the 3 types of electrophoresis

Answer 7

a) plasma proteins electrophoresis
b) polyacrylamide gel electrophoresis
c) isoelectric focusing

Question 8

Describe plasma protein electrophoresis

Answer 8

For separation of plasma proteins

Sample is applied to cellulose acetate solid support

Question 9

Chronic infections

Answer 9

Gamma globulins are increased

Question 10

Gamma globulins ate decreased

Answer 10

Immunodeficiency

Question 11

Liver cirrhosis

Answer 11

Albumin alpha and beta globulins are decreased due to dec liver function

Question 12

Nephrotic syndrome

Answer 12

Inc protein loss in urine all proteins are decreased except a-2 will increase

Question 13

Describe polyacrylamide gel electrophoresis

Answer 13

Polyacrylamide gels cast vertically between a pair of glass plates
After electric current is applied proteins migrate through pores and the smaller the protein the faster it will be

Question 14

Describe isoelectric focusing

Answer 14

Special PAGE where gel is set at a diff PH values
Proteins reach each of their opp charges
Until they reach their isoelectric points

Question 15

Define chromatography

Answer 15

Laboratory technique for separation of mixture in a mobile phase which carries a fixed system called the stationary phase

Question 16

Whats the principal of chromatography

Answer 16

The diff constituents of a mixture have diff affinities for the stationary phase depending on their interactions with its surface so they travel at different velocities causing them to seperate

Question 17

What does chromatography depend on

Answer 17

Nature of stationary phase so mass or charge