Methods Flashcards
0
Q
Annealing
A
Lowering temp to 55*C allowing binding of primers to template DNA
1
Q
Denaturation
A
Heating to 95*C to separate dsDNA
2
Q
Extension/elongation
A
Rise temp to 72*C, Taq fills in template using primer as starting point.
3
Q
Direction of forward primer
A
5’ to 3’
4
Q
Direction of reverse primer
A
3’ to 5’
