medically important yeast Flashcards

1
Q

characteristic of yeast

A

SIMPLE in sporulation and nutritional
ID: sugar assimilation
look for blastospores or produce pseudohyphae

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2
Q

what is yeast plated on for enhancement of sporulation

A

corn meal agar or potato dextrose

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3
Q

how does yeast look on plate

A

creamy, whitish colonies
some are wrinkled and dry

can ID yeast on wet mount

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4
Q

yeast and flora

A

can be normal flora

but can be pathogenic in immunocompromised host

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5
Q

most common yeast

A

candida albicans

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6
Q

Candida albicans infection

A

in oral, vaginal, wound, urinary

can cause thrush- oral infection after antibiotics or chemo- can be systemic

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7
Q

candida albicans is what +

A

germ tube +; outgrowth of organism in pooled human serum

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8
Q

Candida albicans growth

A

mycosel or SABs
production of blastospores, pseudohyphae and chlamydospores

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9
Q

what sugar does Candida albicans assimilate

A

many sugars but MAIN
trehalose

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10
Q

C. dubliniensis is germ tube + but how to differentiate between albicans

A

does not assimilate trahalose

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11
Q

C. tropicalis

A

-UTIs
immunocompromised
virulent in leukemia

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12
Q

C. tropicalis growth

A

produced blastospores either singly or short groups

DOES NOT GROW ON cycloheximide agar (mycosel)

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13
Q

candida kefyr

A

pseudotropicalis

blastospores- logs in a stream

ID on corn meal and seeing blastospores

nail or pulmonary infections

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14
Q

second most candida species to cause infection

A

C. glabrata

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15
Q

C. glabrata main differentiating characteristics

A

ONLY assimilates trehalose and glucose

does not produce pseudohyphae

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16
Q

C. glabrata infection

A

can be systemic and get into blood stream so infections can be aggressive

beginning to show resistance to fungal drugs

17
Q

yeast interest in hospitals

A

candida auris

-spread easily from personnel to patients
-reportable due to high resistance

18
Q

C. auris why serious

A

in some cases resistant to all anti-fungal

serious when isolated in the bloodstream

“superbug”

BIG BLASTOSPORES

19
Q

yeast contaminate around house

A

rhodotorula

-sinks, bathroom

20
Q

rhodotorula infection

A

-orange/salmon color
-contaminant normally

-ONLY isolated from very sick patients -end stage carcinoma or leukemia

-no pseduohyphae

21
Q

baker’s yeast

A

saccharomyces cerevisiae

22
Q

saccharomyces cerevisiae reproduction

A

both sexual and asexual stage and can convert by placing on V8 juice with SABs

rarely source of infection, but increase in HIV pt

23
Q

seen on tomatoes and other fruit

A

geotrichum

machinery mold

associated with lung infections

24
Q

important info about geotrichum

A

grows best at 30 not 37

produces arthrospores that do break apart as gets older

dryish wrinkled colony growing on corn meal

25
Q

common form of yeast meningitis in AIDs patients

A

cryptococcus neoformans

-invade any tissue

26
Q

what harbours Cryptococcus neoforms

A

pigeons and trees

not contagious person to person

from nature

brownish colonies on bird seed agar

27
Q

Cryptococcus neoforms important

A

round yeast- india ink

form a capsule which makes it virulent

urea +

DOES NOT grow on mycosel
-plate CSF on SABs or other nutrient agar

28
Q

known to cause cutaneous skin infection

A

trichosporum beigelli

infection known as white piedra

not common in US

29
Q

infection of trichosporum beigelli

A

superficial infection of hair and can see white nodules along hair shaft

30
Q

how to differentiate between trichosporum beigelli and geotrichum

A

trichosporum beigelli produced arthrospores and blastospores with pseudohyphae

geotrichum- no blastospores

31
Q

yeast that causes pityriasis versicolor

A

Malessezia furfur-normal skin flora

discoloration of the skin

can be dark or light patches on skin

32
Q

Important about Malessezia furfur

A

causative agent of sepsis in people receiving prolonged intravenous lipid therapy

requires long fatty acids for growth so MUST overlay agar with layer of olive oil

spaghetti meatball appearance