media and stains

Question 1

what is SABs primary isolation for

Answer 1

fungus

Question 2

SABs pH

Answer 2

6; makes it inhibitory for some bacteria

basic nutrient media, no antibiotics

Question 3

SABs + blood is good for

Answer 3

sporulation of some of molds

Question 4

BHI+ (brain heart infusion) antibiotics added

Answer 4

cycloheximide and chloramphenicol; keep down bacteria

Question 5

BHI referred to as ??

Answer 5

mycosel plates or BHI with CC plates

Question 6

corn meal/ potato dextrose agar

Answer 6

grows mold and yeast and used for SPORULATION

-enhances pigment of any fungus

Question 7

birdseed agar

Answer 7

• Detect presence of Cryptococcus neoformans
  ○ Produce brown pigment

Question 8

chromager

Answer 8

• Really used for ID yeast
  
  • Cornmeal base to which indicators have been added allowing different yeast types to produce different colors

Question 9

KOH Prep

Answer 9

• Determine presence of a fungal element on hair, skin, and nails
  
  • Placed in KOH solution and sit for 15-30 min in order to dissolve keratin
    ○ Allow to see fungal element
  • ONLY tells you if something is present

Question 10

calcofluor white stain

Answer 10

• Fluorescent stain that can be used in place of KOH; more sensitive
  
  • Fungal elements in hair, skin, and nails
  • Stain binds to chitin on fungal element allowing to fluoresce

Question 11

india ink prep

Answer 11

• Detect large capsule Cryptococcus neoforms
  ○ Placed on slide and India Ink dropped onto specimen and viewed

Question 12

gomori silver nitrate stain

Answer 12

• Detect presence of Pneumocystis jiroveci
  
  • # 1 cause of death in HIV/AIDS patients
  • Common specimen = lung tissue

Question 13

Periodic-Acid-Schiff Stain (PAS

Answer 13

• Detect presence of fungal elements in vivo (tissue specimen, biopsies, etc.)
• Not used in clinical lab
• Fungal elements will stain pink or purplish in specimen

Question 14

Lactophenol Cotton Blue (LPCB)

Answer 14

• Used to help visualize fungus under microscope
  
  • Helps visualize components better, see different structures
    Used in vitro

Question 15

gold standard for looking at sporulation structures

Answer 15

slide culture technique

Question 16

slide culture technique

Answer 16

enhance sporulation of fungus/ molds

square of PDA or CMA
-piece of growing fungus placed on 4 corners with teasing needle and place coverslip on top

-incubate, and after a couple weeks place coverslip into LCPB to view structures

Question 17

scotch tape prep

Answer 17

• Take clear piece of scotch tape, place into a growing fungi and then place into LPCB

Question 18

teasing the fungus

Answer 18

• Tease off growing colony with needle
  Need to sterilize in between uses

Question 19

yeast API

Answer 19

• Yeast Identified by sugars utilized
  
  • If uses sugar turns turbid
  • ONLY yeast, no mold
    Sugar assimilation (means uses the sugar)