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MCB-L > Media, Methods of Isolation, & Aseptic Transfer > Flashcards

Media, Methods of Isolation, & Aseptic Transfer Flashcards

1
Q

Selective Media

A

favors growth of ONE group of micro-organisms & inhibits growth of others - usually gram - or +

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2
Q

Differential Media

A

Allows you to distinguish among the bacteria in a sample by there ability to utilize certain substrates or perform certain reactions

Contains an indicator to detect presence or absence pf specific metabolic activity (e.g. lactose fermenters) which is usually indicated by a color change (allows for distinguishing among types)

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3
Q

Mixed & Pure Cultures

A

mixed cultures consist of two or more species

pure cultures consist of only ONE species

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4
Q

Coliform bacteria

A

normally found in large intestine or aquatic environments, soil, and on vegetation

universally present in feces of warm-blooded animals

bacterial indicator of sanitary quality of food & waater

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5
Q

Coliform bacteria includes

A

E. coli (fecal coliforma) , Enterobacter, & Serratia

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6
Q

Presumptive Identification

A

Tentative identification of an isolate based on one or more key test results

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7
Q

CFU (Colony Forming Unit)

A

general term used to describe the origin of a colony

e.g. some colonies form from individual cells & others from pairs, chains, or cluster of cells

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8
Q

Growth Media

A

Common media used for maintaining bacterial cultures

Must meet the diverse nutrient requirements of routinely cultivated bacteria

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9
Q

Types of growth media

A

General-purpose media

Enriched media

Selective and differential media

Undefined (complex) media

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10
Q

General-purpose media

A

used to cultivate microbes

e.g. trypticase say agar

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11
Q

Enriched media

A

contains special growth factors that certain species must be provided to grow

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12
Q

Selective and differential media

A

used in identification of microbes

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13
Q

Undefined (complex) media

A

the exact composition and amounts of carbon and nitrogen are not known

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14
Q

Forms of media

A

nutrient broth and nutrient agar

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15
Q

Nutrient broth

A

liquid form

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16
Q

Nutrient agar

A

solid form

agar slate, agar deep, & agar plate (Petri dish)

17
Q

Agar slants

A

allowed to cool and solidify on an angle

18
Q

Agar deeps

A

allowed to cool and solidify in upright position

19
Q

Agar plate (Petri dish)

A

a lid is used to prevent airborne contamination

plates must be stored or incubated upside down to prevent condensation droplets that may form on the lid

20
Q

Aseptic transfer technique

A

techniques utilized during transfer of sample (inoculum) to sterile culture medium

prevent contamination of sample (source), sterile culture medium, or surroundings

21
Q

Inoculating loops

A

used to smear, streak, or retrieve an inoculum from a culture of micro-organisms

22
Q

Identify whole colony features

A

round
irregular
spindle
filamentous
rhizoid

23
Q

Identify margin features

A

smooth
irregular (erose)
lobate
filamentous (filiform)
rhizoid

24
Q

Aseptic transfer from broth to broth

A
25
Q

Aseptic transfer from broth to slant

A
26
Q

Aseptic transfer from source plate to sterile agar plate (streak plate method of isolation)

A
27
Q

Note for streaking

A

during streaking, the cell density decreases, eventually leading to individual cells being deposited separately on the agar surface

cells that have been sufficiently isolated will grow into colonies consisting ONLY of the original cell type

28
Q

Identify

A

Completed streak plate

Each streak takes a little from the previous quadant, spreads it to the next and then transfers some to the next before finally spreading in the 4th quad for isolated colonies

Thickest growth occurs in the first quadrant streak and isolated colonies occur on the fourth quadrant streaked

29
Q
A

Step #1 (thickest growth)

30
Q
A

Step #2

31
Q
A

Step #3

32
Q
A

Step #4 (isolated colony)

MCB-L (12 decks)

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