Match the tube to the test

Question 1

Tubes 3 & 4 Reagent?

Answer 1

Urea Hydrolysis/ Urease test Urease and Phenol Red 3(+), 4(-)

Question 2

Tubes 1 & 2 Reagent?

Answer 2

ONPG test onpg disk 1(+), 2(-)

Question 3

Plate 7 & 8 Reagent?

Answer 3

Starch Hydrolysis test Iodine 7(+), 8(-)

Question 4

Tubes 5 &6 Reagent?

Answer 4

Phenylalanine Deaminase test Ferric Chloride 5(+), 6(-)

Question 5

Tubes 9 & 10 Reagent?

Answer 5

MR test Methyl Red 9(+), 10(-)

Question 6

Tubes 19 & 20 Reagent?

Answer 6

Tryptophan Hydrolysis/ Indole Production test Kovac’s reagent 19(+), 20(-)

Question 7

Tubes 17 & 18 Reagent?

Answer 7

Simmon’s Citrate Bromthymol Blue 17(+), 18(-)

Question 8

Tubes 11 & 12 Reagent?

Answer 8

VP Test Alpha-napthol and KOH 11(+), 12(-)

Question 9

Tubes 15 & 16 Reagent?

Answer 9

Decarboxylation Test Bromocarbol Purple 15(+), 16(-)

Question 10

Tubes 21, 22, 23, 24

Answer 10

Kliger’s Iron Agar (KIA) 21 (Glu+, Lac+, Gas+, H2S-) 22 (Glu+, Lac-, Gas-, H2S+) 23 (Glu-, Lac-, Gas-, H2S-) 24 (Glu+, Lac-, Gas-, H2S-)

Question 11

Tubes 29, 30, 31 Determine positive?

Answer 11

Nitrate Reduction Test 3 Steps: Observe bubbles, reagent: Sulfanilic acid and alpha naphthylamine, Zinc 29 Bubbles+ 30 No Bubbles, Reagent turned red + 31 No bubbles, Reagent (no change), Zinc Turned rose-

Question 12

Tubes 25, 26, 27, 28

Answer 12

O-F test 25(+oxid, +glu aerobic) 26(+Ferm, +glu anaerobic) 27(+oxid aerobic) 28(-Ferm)

Question 13

Tubes 32, 33, 34

Answer 13

Sugar Fermentation test 32 (Bubbles+, Acid+) 33 (Bubbles-, Acid+) 34 (Bubbles-, Alkaline reaction)

Question 14

Catalase Test?

Answer 14

Identifies organisms that produce the enzyme catalase. Reagent: Hydrogen Peroxide Positive result= bubbles

Question 15

Oxidase test?

Answer 15

Identifies bacteria containing the respiratory enzyme cytochrome oxidase Reagent= oxidase