Final note cards

Question 1

Obligate aerobes?

Answer 1

(growth only in the top portion of tube where O2 is) Bacteria that must have oxygen for survival

Question 2

Facultative anaerobes?

Answer 2

(Uneven distribution of growth from top to bottom more at top) -Prefer to utilize oxygen, but can also use sources as nitrate for anaerobic respiration.

Question 3

Microaerophiles?

Answer 3

Prefer conditions with lowered oxygen content and usually increased levels of CO2.

Question 4

Thioglycollate?

Answer 4

Supports all types of bacterial growth. Suggest which type of environment a bacteria favors.

Question 5

Reazurin?

Answer 5

A indicator that turns pink in the presence of oxygen. Seen at the upper portion of the test tube.

Question 6

Gas-Pack Jars?

Answer 6

These jars provide a completely anaerobic environment. With a indicator for oxygen (blue= + for oxygen)

Question 7

obligate anaerobes?

Answer 7

(Absence of growth in the top portion of broth) - Dies where O2 is

Question 8

Aerotolerant anaerobes?

Answer 8

(Uniform growth from top to bottom ) Oxygen has no effect.

Question 9

What type of growth is seen?

Answer 9

Obligate aerobe

Question 10

What type of growth is seen?

Answer 10

Aerotolerant anaerobe

Question 11

What type of growth is seen?

Answer 11

Facultative anaerobe

Question 12

What type of growth is seen?

Answer 12

Microaerophiles

Seen at and right below the pink oxygen indicator line

*USE CAUTION*

easy to mess up

Question 13

Candle Jar is used for what?

Answer 13

To increase carbon dioxide levels and to decrease oxygen. These are used best with Microaerophiles.

Question 14

Mueller-Hinton agar

(MHA)

Used for?

Answer 14

To prevent inhibition of some antibiotics

Question 15

Stapylococci and Streptococci are referred to as?

They are gram?

and usually have a shape of?

Answer 15

Pyogenic (pus-forming)

Gram +

Cocci

Question 16

Staph or Strep?

How can you tell?

Answer 16

Staphylococci

They divide in more than one plane forming irregular clusters.

Question 17

Staphylococcus or Streptococcus?

How can you tell?

Answer 17

Streptococci

They divide to form long chains

Question 18

What type of enviornment do Streptococci perfer to live in?

Answer 18

Microaerophilic

Question 19

Coagulase test

checks?

Answer 19

If the bacteria have the enzyme “Coagulase” which allows the bacteria to form clots from the fibrin in the blood.

Question 20

What test is this?

The purpose?

What did it check?

Answer 20

Mannitol Salt agar

Differential test (Produce observable differences)

*Salt inhibits many bacteria, fernmentation of the mannitol will cause the medium to become acid and color change to yellow.

Indicator-phenol

Question 21

The Bile esculin test?

Why would you use this test?

Positive result?

Answer 21

Identifies bacteria able to hydrolyze esculin in the presence of bile.

Used for a presumtive identification of group D streptococci and enterococci (both +)

After 72 hours of incubation if more than half of tube is black then +

Question 22

CAMP Test: Used for?

Positive result?

Answer 22

Used for presumptive identification of group B streptococcus (Strep Agalactiae) the only beta-hemolytic strep that gives (+)

Positive result- See a arrowhead hemolysis between the junction of growth of S. aureus and group B strep.

Question 23

Enterobacteriaceae family

characteristics?

Answer 23

Gram Negative, non-spore forming, facultatively anaerobic bacilli or coccobacilli

Question 24

6.5% NaCl Broth

Salt Tolerance Test

Used for?

Answer 24

Separates Enterococcus from streptococcus

Positive result gives Enterococcus Group D

(Prelimarary test)

Question 25

Eosin Methylene Blue Agar

Used for?

Answer 25

A selective and differential agar

Allows quick indication of gm(-) enteric pathogens.

&

Green sheen = Lactose fermenters

Question 26

Cloumbia agar

“CNA”

?

Answer 26

Selective for gram positive bacteria

Blood plates with antibiotics against gram negative