MAPs, MTs and force generation

Question 1

Overview of MT growth and shrinkage

Answer 1

Rapid growth with GTP cap
accidental loss of GTP cap
rapid shrinkage
regain of GTP CAP
rapid growth with GTP cap

Question 2

What are MAPs

Answer 2

Microtubule associated proteins that regulate MT’s stability and function

Question 3

What do Dam1/DASH do

Answer 3

In yeast and fungi
Microtubules attach to the chromosome pairs through protein complexes called kinetochores. During cell division, microtubule depolymerization pulls the chromatids apart. The DASH/Dam1c complex, forms a ring around a microtubule to organize the kinetochore-microtubule

Question 4

What does MAP65 do?

Answer 4

Binds to interpolar antiparallel MT’s forming bridges which stabilises spindle midzone region or mitotic spindle.

Question 5

Two types of motor proteins for microtubules

Answer 5

Kinesin - + end

Dynein - - end