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CEDB30002 Cell Biology > Mammalian Genetic Manipulation > Flashcards

Mammalian Genetic Manipulation Flashcards

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1
Q

Chimera

A

Cell populations from multiple embryos (Inject ES to blastocyst)

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2
Q

Cre-Lox System

A

Tissue specific gene targeting.
Tissues specific promoter and Cre-recombinase (enzyme only made in certain tissue).
Will interact with floxed GOI to knock it out.

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3
Q

Floxed Gene

A

Has LoxP flanking sequence and is removed as circular DNA due to action of Cre recombinase.

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4
Q

Homologous Recombination

A 
Similar genetic material.
Inactivate gene X.
Only NEO is present (resistant to G418)
TK has been lost from end (resistant to Ganciclovir)
Live Cells
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5
Q

Heterologous Recombination

A

Different genetic material. Random integration.
Neo and Tk present (G418 resistant, Ganciclovir sensitive)
Dead Cells

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6
Q

Inner Mass Cells Fate

A

Become ES cells

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CEDB30002 Cell Biology (10 decks)

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