Agrobacterium Infection Flashcards
1
Q
VirA
A
Sense acetosyringone/wound signals. Autophosphorylate, phosphorylates and activate VirG.
2
Q
VirG
A
Transcription factor. Activates Vir genes when activated by VirA.
3
Q
VirC
A
T-DNA processing.
4
Q
VirD1
A
Binds TDNA borders. Recruits VirD2.
5
Q
VirD2
A
Relaxase. Nicks TDNA borders, covalently attaches to 5’ end of T strand. Target T-DNA to NLS
6
Q
VirE2
A
ssDNA binding protein. Protect DNA.
7
Q
VirB
A
Transmembrane pore.
8
Q
VirF
A
Goes to host nucleus. Associates with host cell proteins, makes E3 ligase, ubiqutinates VirE2 so integration can occur.
9
Q
Octopine
A
Pyruvic acid group
10
Q
Nopaline
A
Alpha-keto glutaric acid group
11
Q
VirD4
A
Recruits DNA-protein complexes for T-complex transfer
