M104 T2 L9 Flashcards
What states does the human metabolism oscillate between?
the fed and fasting states
What is the ‘switch’ that determines metabolic changes?
the molar ratio of insulin to glucagon in the blood
When is the FED metabolic state in place?
during meals and for several hours afterwards
What are the FED and fasting metabolic states characterized by?
FED - high insulin and low glucagon (a high insulin/glucagon ratio)
fasting - low insulin and high glucagon (a low insulin/glucagon ratio)
When does the fasting metabolic state kick in?
6-12 hr after a meal
What is the criteria of ‘prolonged fasting’ or starvation?
fasting that lasts in excess of 12 hrs
What is the effect of food intake (FED state)?
stimulates insulin release
insulin inhibits glucagon secretion
this affects metabolism in the liver, muscle and adipose tissue
However, glucose utilization in the brain remains unchanged
What is the effect of high Cs of nutrients on the the insulin:glucagon ratio?
causes it to increase
What happens when there is a high blood glucose?
it enters the liver and is converted to glycogen and TGs
these are secreted as VLDL
some enters TCA cycle
What is glycerol from peripheral tissues converted into?
triacylglycerols
What happens to excess amacs entering from the gut?
they are converted to pyruvate and metabolised via the TCA cycle for energy or converted to triacylglycerols
How does glucose enter muscle tissue?
via the insulin-stimulated Glut 4 transport system
What happens to glucose once it has entered the muscle tissue?
it is converted to glycogen or metabolised via glycolysis and TCA cycle
How do FAs enter muscle tissue?
via chylomicrons (if from the diet) via VLDL (if from the liver) via VLDL. These
What are the two sources of FAs?
the diet
the liver
What happens to FAs once they enter muscle tissue?
they are oxidised via β-oxidation to acetyl CoA to produce ATP to support contraction
How does glucose enter adipose tissue?
via the insulin-dependent Glut 4 transport system
What happens to glucose once it enters adipose tissue?
it is converted into acetyl CoA and PDH via glycolysis
glucose < acetyl CoA and PDH < FAs < triacylglycerol
How do FAs enter adipose tissue?
via VLDL and chylomicrons
What happens to FAs once they enter adipose tissue?
it is converted to triacylglycerol
What happens to glycerol released from TGs?
it is returned to liver for re-use
What is the effect of insulin activity on LPL and HSL
activity?
LDL - is increased
HSL - is reduced
How does the brain take up glucose?
via Glut 1 & 3 transporters
What happens once glucose enters the brain?
it metabolises it oxidatively by glycolysis and the TCA cycle to produce ATP
How does the role of the liver change during fasting?
switches from a glucose-utilizing to a glucose-producing organ
there is a decrease in glycogen synthesis and an increase in glycogenolysis
What happens as plasma glucose falls?
glucose no longer enters liver as Glut 2 transporter has low affinity
What is the effect of a reduced insulin: glucagon ratio?
it activates glycogenolysis and gluconeogenesis (from lactate and alanine) via cAMP production in response to glucagon
What are FAs from lipolysis used for?
to produce E via b-oxidation
What is citrate and acetyl CoA produced from?
from the oxidation of FAs - this activates gluconeogenesis and inhibits glycolysis
What is the effect in reduced insulin levels?
it reduces glucose entry into muscle tissue
glycogenolysis does not occur as there are no glucagon receptors in skeletal muscle to cause activation
When insulin levels are low, what is used as an alternative E source?
muscle and other peripheral tissues switch to FA oxidation as a source of E
this inhibits glycolysis and glucose utilisation
In the early fasting state, what happens to proteins
they are broken down to amacs
the carbon skeletons can be used for E
or exported to the liver in the form of alanine
What is the entry of glucose into adipose tissue via the Glut 4 transport system reduced in response to?
in response to the lowered insulin and metabolism of glucose via glycolysis being severely inhibited
What does the mobilisation of TGs occur in response to?
in response to the reduced insulin:glucagon ratio and activation of the sympathetic NS by release of noradrenaline
What are FAs used for in adipose tissue?
used directly within the tissue to produce energy
the remainder are released into the bloodstream to support glucose-independent E production in muscle and other tissues
What happens to glycerol in adipose tissue?
it cannot be metabolised so it is recycled to the liver to support gluconeogenesis
How does the brain continue to take up glucose in the early fasting state?
bc of the high affinity of Glut1 and Glut3 transport system and independence from insulin
so glucose continues to be metabolised despite the fact that no glucose is provided in the diet
Why does the brain continue to take up glucose in the early fasting state rather than using alternatives?
bc the brain can’t switch to FAs as a source of fuel as free FAs don’t cross the blood brain barrier