M: Virology 3 - Week 11 Flashcards
Name 2 DNA viruses that can cause conjunctivitis
Adenovirus
Molluscum contagiosum
(also HSV and VSV but they are less important to remember)
Name 2 RNA viruses that can cause conjunctivitis
Enterovirus 70
Coxsackie virus 24
What type of cconjunctival infection is the most common?
viral conjunctivitis
Where does viral conjunctivitis typically occur? (i.e. source of the infection)
Upper respiratory tract
Are lymphoid follicles common or rare in viral conjunctivitis?
common
Where do adenoviruses start? Where can they spread to and replicate? (3)
Start in glandular tissue. Can spread to conjunctiva, pharynx, GIT
In regards to Adenoviruses, describe:
A: Capsid symmetry
B: Are they enveloped or non-enveloped?
C: Where are their fibres located?
A: Icosahedral
B: non-enveloped. Therefore are hardy
C: at their apices
What conditions can adenovirus cause? (4)
ocular conditions (e.g. conjunctivitis)
respiratory
genital
GIT disease
What virus is considered the most common cause of viral conjunctivitis?
Adenoviruses
How may adenovirus ocular infections present? (2) What adenoviruses are responsible for each presentation?
Pharyngeal conjunctival fever (PCF) - adeno 3, 4, 7 Epidemic keratoconjunctivis (EKC) - adeno 8, 19, 37, others
Why are adenoviruses difficult to disinfect against?
Because they are non-enveloped
Describe the following features of adenoviruses:
D: how contagious are they?
E: how robust are they?
F: how long is their incubation period?
D: Highly contagious
E: Highly robust; survives in environment
F: Incubation period = 4-18 days long
Describe the following features of adenoviruses:
G: How long do they survive on fomites?
H: Where are particles shed from (2) and for how long? (1)
G: 8-35 days
H: particles are shed from nasopharynx and tears for 2 weeks
List 3 (out of the 11) symptoms of epidemic keratoconjunctivitis (EKC)
frequent dull pain, photophobia, reduced VA [important]
preauricular or submandibular adenopathy [important?]
significant periorbital lid edema
Marked conjunctival hyperemia, chemosis
Petechial haemorrhages common
Superficial punctate keratitis
Subepithelial infiltrates 1-2 weeks after onset
Why can epidemic keratoconjunctivitis (EKC) present with submandibular adenopathy?
because adenoviruses are also replicating in glandular tissue such as submandibular glands
How long after the onset of epidemic keratoconjunctivitis (EKC) do subepithelial infiltrates occur?
1-2 weeks after onset
How long may subepithelial infilrates last in people with epidemic keratoconjunctivitis (EKC)?
weeks or months
What age group is pharyngeal cconjunctival fever (PCF) predominantly seen in?
children
What are midwinter epidemics of PCF due to? (1)
respiratory infections
What are summer episodes of PCF associated with?
swimming pools
Describe the following features of PCF:
A: unilateral or bilateral?
B: list 6 other symptoms
A: can be either unilateral or bilateral B: tearing fever preauricular adenopathy pharyngitis occasional superficial punctate keratitis subepithelial infiltrates
In terms of preauricular adenopathy, how are EKC and PCF differentiated?
EKC: is tender
PCF: is not tender
Describe the pathogensesis (in terms of replication) of adenoviruses in 4 steps
- Bind well to CAR on mucosal epithelium
- Enter cell via RME through clatherin coated pits
- Acidification in the endosome modifies the capsid proteins
- This allows release into cytoplasm and transport via microtubules to the nucleus for replication
What does CAR stand for?
cell adenovirus receptor
What contributes to the pathogenesis of adenovirus after replication? (3)
Release of new virions result in cell lysis (i.e. a cytotoxic virus)
Some gene products suppress killing by cytotoxic T cells, so infection persists, stimulating large infiltrates of T and B cells
Even after infection is cleared, persistant infiltrates may lead to loss in VA for weeks to months
How can we manage adenovirus infections? (4)
Supportive treatment (compresses, other pain relief, etc.) Instruct re-scrupulous handwashing to prevent spread Anti-inflammatory agents to reduce infiltrates (e.g. NSAIDs) Topical steroids (controversial, but consider for severe EKC)
Are there any specific anti-virals for adenovirus infections?
No
In regards to Enteroviruses, describe the following:
A: What family of viruses do they belong to?
B: What is their capsid symmetry?
C: How big are they?
D: Are they enveloped or non-enveloped?
A: picornaviridae
B: Icosahedral
C: Small
D: Non-enveloped
Name the 2 subtypes of enteroviruses that are ocular pathogens
Enterovirus 70
Coxsackie virus 24
In what age groups do we see enterovirus infections? (2)
children and young adults
In regards to Enteroviruses, describe the following:
E: How contagious are they?
F: How long is their incubation period?
G: How are they transmitted? (2)
E: Highly contagious
F: Rapid (24 hours)
G: Fingers and Fomites (i.e. optometrists)
What proportion of enterovirus infections involve subconjunctival hemorrhages?
80%
Describe the course and recovery of enteroviruses (2)
rapid course with uneventful recovery
Can enterovirus infections present with a severe presentation?
yes
What symptoms do enterovirus infections present with? (3)
Sensation of burning or foreign body
Eyelid swelling
Photophobia