Locating Sequences

Question 1

N-terminal hydrophobic alpha helix

Answer 1

Mitochondria

Question 2

Lysine/arginine rich sequence

Answer 2

Nucleus

Question 3

SKL

Answer 3

Peroxisome

Question 4

Stop transfer sequence

Answer 4

Plasma membrane

Question 5

Mannose 6 phosphate

Answer 5

Lysosomes

Question 6

Trp-rich domain

Answer 6

Secretory vesicles

Question 7

KDEL

Answer 7

ER retention. Usually leave ER and then return.

Question 8

Positively charged N terminal

Answer 8

For the ER

Question 9

Which pathways are cytoplasmic?

Answer 9

Cytoplasm, mitochondria, nucleus, and peroxisome

Question 10

Which signals are secretory?

Answer 10

Plasma membrane, lysosomes, secretory vesicles, ER

Question 11

Mitochondrial transport

Answer 11

Interact with HSPs because the protein has to unfold to enter the TOM/TIM to get into the matrix

Question 12

Nuclear transport

Answer 12

Cytoplasmic. Happens via nuclear pores and if they are large they will require nuclear localization signals — 4 continuous basic residues like Lys and Arg

Question 13

I-Cell disease

Answer 13

Lysosomal storage disease where M6P is defective. High levels of lysosomal enzymes.