Liver, Gallbladder & Pancreas VM Flashcards
What do hepatocytes line (or separate)?
Hepatocytes separate the space of disse from the bile canaliculi.
What are the large cells featured?
What are the prominent space between these cells?
Hepatocytes (note the granularity, and some are binucleate)
These are probably sinusoids based on the number of erythrocytes present.
From what cell lineage are kupffer cells derived, and what are their functions?
Monocytes; serve to endocytose particles & cell debris.
What type of epithelium lines the lumen of a bile ductule?
Simple cuboidal
How do glycogen and lipid granules stain on MBBF (methylene blue basic fuschin)?
Glycogen granules stain darkly (blue)
Lipid droplets stain pink & “foamy”
What types of intercellular junctions are found at the margins of the canalicular area of adjacent hepatocytes? Why?
Gap junctions (function as a syncytium) and tight junctions (sequester the now water-soluble conjugated bilirubin to the canaliculi)
What type of collagen makes up the reticular fibers found in the liver (and spleen, lymph nodes, etc…)
Type III Collagen
Distinguish between the free and attached surfaces of the gallbladder on histology.
What are the functions of the gallbladder?
Free wall has a serosa, attached has an adventitia.
To store, concentrate, and release bile.
What type of epithelium lines the gallbladder?
What does the presence of mucosal folds indicate?
Simple (ciliated) columnar.
That the gallbladder wasn’t distended upon fixation.
The basal cytoplasm of a pancreatic acinar cell is (basophilic/eosinophilic) due to the presence of ____, while the apical cytoplasm is (basophilic/eosinophilic) due to the presence of ____.
Basal is basophilic (rER)
Apical is eosinophilic (zymogen granules)
Describe the hierarchy of ducts moving from proximal to distal.
What are they lined with?
Intercalated ducts > Intralobular ducts > Interlobular ducts > Main pancreatic duct
Lined by centroacinar cells, distally surrounded by connective tissue.
This is a trichome stain of ____ at what stage?
Liver cirrhosis at stage 3-4 (note “bridging” fibrosis).
