LIFBCWCD Flashcards
What is involved in an FBC?
→Hb: concentration of Haemoglobin →Hct: Percentage of blood volume as RBC →MCV: Average size of RBC →MCH: Average haemoglobin content of RBC →RDW: Range of deviation around RBC size →Reticulocyte count →Blood film
What are the WBC results in FBC?
→Total WBC and differential
→Neutrophils, lymphocytes, monocytes,
basophils, eosinophils
→Platelet count and size
What is polychromasisa?
→Polychromasia- bluish colour due to increased RNA
What are abnormal cells of WBC?
→blasts,
→atypical lymphoid cells
How is FBC processed?
→using optical scatter methods
How is FBC presented?
→cytogram where population of cells are displayed in distinct cluster.
What happens if the differentials are not displayed correctly?
→alerts operator to refer the sample for a blood smear to be examined
What are the advantages of automated hematology analyzer?
→Efficient and cost effective
→Accuracy and precision of quantitative blood test
→Ability to perform multiple test on single platform
→Reduced labour requirements
→Invaluable for accurate determination of red cell indices; e.g. MCV
What are the disadvantages of automated hematology analyzer?
→Flagging of laboratory test results demand labour intensive manual examination of blood smear
→Comments on red cell morphology cannot be generated.
→Platelet clumps are counted at single, so low count.
→Expensive with high running cost
What are the morphological features of macrocytic megaloblastic anaemia?
→Large RBC, different shapes
→Increased Hb
→Vit B12 deficiency as a result of IF because removal of stomach.
What are the morphological features of acquired haemolytic anaemia?
→No bioconcave
→Increased RNA due to increased haemolysis
→polychromasia
→spherocytes
What is present in SCA blood film?
→Target cells
What are the morphological features seen in myelofibrosis?
→Tear drop poikilocytosis
What are the morphological features of neutrophil leucocytosis?
→Increased neutrophils
→RBC are smaller and paler
→Prickle cells present
→Microcytic hyperchromic
What are the morphological features of acute myeloblastic leukaemia?
→Immature myeoloids present
→Cant see any platelets