Lesson 5 - Collection Tubes And Equipment Flashcards
Which collection tube has additive EDTA?
A. yellow
B. lavender
C. red/red-speckled
D. gold
B. Lavender
What does inversion mean to a phlebotomist?
Inversion means to gently tilt, shake, or bounce your collection tube or object.
Which color collection tubes require 8-10 inversions?
Hint: it’s 4 different colored tubes
Yellow, White, green, lavender, and gray tubes
Which colored collection tubes require the phlebotomist to invert them 5 times?
Hint: it’s 1 color tube
A Green
B Yellow
C Red/red-speckled
D Light blue
C. Red and red-speckled tubes
Which colored collection tube requires the phlebotomist to invert it 3-4 times?
Hint: it’s 1 color tube
Light blue colored collection tubes
What is the order of blood drawing for the colored coded collection tubes?
Hint: it’s 1-7 colored tubes
- White topped tubes
- Yellow topped tubes
- Light blue topped tubes
- Red topped tubes
- Gold or red-speckled topped tubes
- Green topped tubes
- Lavender topped tubes
- Gray topped tubes
The red collection tube is used to collect what?
Serum from the blood
What is the acronym to help remember the order of collection is?
Hint: YLRGGLG
Yesterday
Lisa
Ran
Great
Gallops
Like
Giselles
Why are plastic tops placed on collection tubes?
To prevent and minimize aerosol formation inside the tube when the needle is being removed
What is the materials that most collection tubes are made out of?
Shatterproof Glass or plastic
Which size needle is the biggest?
Hint: remember, the larger the size or number the smaller the needle
A. 5 G
B. 10 G
C. 15 G
D. 22 G (gauge)
Answer: A - 5 G
About how much blood can collection/evacuation tubes hold?
2-15 mLs (milliliters) or blood
What are the parts of blood used for analysis?
Hint: it’s 3 parts of the blood
Whole blood, serum, and plasma
When a phlebotomist draws a whole blood sample, what is it mixed with in its collection tube?
An anticoagulant to prevent clotting
What can whole blood be used to test for in hematology?
Hint: it’s can test for 4 things
Blood type, blood cells, hormone levels, and metal levels
What is serum? How can you get it?
The fluid that sits on top of the blood sample after it has separated from the blood. Can be centrifuged to separate from the plasma or it can be sat out for about 20-60 seconds
What can serum be used to test for in most chemistry tests?
Hint: can test for 2 things
Hint 2: on the periodic table
Sodium and potassium levels
What is prothrombin time (PT)?
What amount of time it takes for your blood to clot.
What can plasma be tested for?
A. complete blood count (CBC)
B. prothrombin time (PT)
C. STAT chemistry tests.
D. All of the above
D. All of the above
Why is order of draw important?
The Order of Draw prevents additives from one tube from causing an error in testing of another tube due to mixing of additives.
Which agency mandated the standard of the Order of Draw?
CLSI or the Clinical Laboratory Standards Institute
Red, gray, and gold tubes contain which additive that enhance the process of normal clotting?
Hint: start with an “S”
Silicone
What are the types of additives collection tubes can hold?
Hint: there are 3 additives
- Anticoagulants - prevent clotting
- EDTA - acid to prevent clumping of platelets
- Clot activators - promote blood clotting
What does SST stand for in blood collection tubes and what kind of additive does it contain?
Serum separator tubes with polymer gel
Describe the yellow topped tubes
-Order of draw, what it’s used for, # of inversions, and type of tube additive if any-
- Is a sterile tube so it is Drawn 1st in order of draw
2.Can be used for blood cultures - Inverted 8-10 times
- Additive is an anticoagulant also known as SPS
What are green topped tubes used to test for?
Hint: tests for 4 things
Ammonia, electrolytes, ABGs (arterial blood gases), STAT or routine chem tests
What is the acronym used for the tubes colors in the Order of Draw?
YESTERDAY - Yellow
LISA - Light blue
RAN - Red
GREAT - Gold and red-grey speckled
GALLOPS - Green
LIKE - Lavender
GISELLES - Gray
What are gray topped tubes used to test for?
Hint: tests for 3 things
Glucose levels, lactic acid, blood, and alcohol levels
How many degrees does a phlebotomist invert the collection tubes?
A phlebotomist must invert all filled collection tubes a full 180 degrees as soon as possible to thoroughly mix the blood with the additives in each tube
What antiseptics are used to cleanse the drawing site?
Hint: there are 3 types
- 70% isopropyl alcohol
- Betadine/povidine-iodine (10%)
- chlorhexadine gluconate (chlorhex)
Finish the sentence: “the larger the needle gauge, the ____ the diameter of the needle opening.”
Smaller
Which antiseptic is used for patients receiving a blood culture but are allergic to betadine?
Cholrhexadine gluconate is the substitute used in place of betadine.
Alcohol can be used to cleanse the drawing site for patients getting a blood culture test.
True or false?
False, blood culture tests requires betadine and/or chlorhexadine gulconate as a substitute
Which type of additive is contained in a lavender topped tube as the anticoagulant and is inverted 8-10 times?
EDTA
The phlebotomy technician receives an order to collect blood culture and glucose, which tube is drawn first?
Yellow for blood cultures first then gray for glucose is second
Reference: (YellowLRGGLGray)
If the lab test ordered is coagulation studies which tube will be used for blood coagulation?
Light blue tubes are used for coagulation tests with the additive sodium citrate.
After the venipuncture is complete, the phlebotomist must clean the site and blow on it to dry.
True or false?
False, after cleansing the site with an alcohol pad it must air dry
What are the chemicals added to evacuated tubes called?
Additives
What is an agent that prevents growth of bacteria called?
Bacteriostatic
What is a substance that prevents infection called?
An Antiseptic
What are additives that stimulate clotting called?
Clot additives/activators
What is an agent used to clean surface other then living tissue called?
A disinfectant
What is the number describing the diameter of a needle’s lumen?
The gauge
What is a hollow tube within a needle called?
A lumen
What are patients admitted into the hospital called?
Inpatients
What would the advice be for adapting a butterfly needle to an evacuated tube?
Use a lure adapter
What is a translucent plastic cylinder connecting a multi sample needle to an evacuated tube called?
A needle adapter
What is the prescribed sequence in which tubes with different additives should be filled during a multitude collection called?
The Order of Draw
What are patients treated at a hospital without being admitted as an inpatient called?
Outpatients
What is an inert additive used to separate cells from plasma during centrifugation called?
Hint: it is also known as thixotropic gel
Polymer gel
What is an inert additive used to separate cells from plasma during centrifugation
Hint: it is also known as polymer gel
Thixotropic gel
What is the rubber and elastic strip of material used to reduce circulation and increase the prominence of veins called in phlebotomy?
A tourniquet
What is the mark on a needle adapted indicating how far the tube can be pushed without losing vacuum seal called?
The Tube Advancement Mark
A translucent plastic connecting the needle to the evacuated tube called?
Hint: This is also known as a needle adapter
A tube holder
What is a blood sample called before it is separated?
Whole blood
What is a small needle and flexible tube for delicate veins called?
Hint: this is also known as a butterfly needle
A winged infusion set
What types of blood draws requires Betadine/Povidone-iodine as the antiseptic cleanser for cleaning the site of injection?
Hint: there are only 3
Blood cultures, arterial punctures, & alcohol level draws
What is the antecubital space on your arm?
The crease between your forearm and bicep or where your arm bends at the elbow
What are the three parts of a needle?
The hub, shaft, and lumen
