LESSON 4a: Microscopy

Question 1

Any microscope that uses light to observed a species.

Answer 1

Light microscopy

Question 2

Enables us to observe small things to the naked eye.

Answer 2

Microscopy

Question 3

Metric unit of microscopy in 10^6 or 1,00,000

Answer 3

micrometer (um)

Question 4

Metric unit of microscopy in 10^9 or 1,000,000,000

Answer 4

nanometer (nm)

Question 5

Metric unit of microscopy in 10^10 or 10,000,000,000

Answer 5

angstrom (A)

Question 6

Concentrates light to the specimen.

Answer 6

Bright field microscopy

Question 7

Can use stains to increase contrast, when light concentrates to the specimen.

Answer 7

Bright field microscopy

Question 8

Causes light to reflect off the specimen, causing dark background.

Answer 8

Dark field microscopy

Question 9

What light microscopy should be used in observing live cell, or unstainable cells?

Answer 9

Dark field microscopy
Normarski microscopy
Phase contrast microscopy

Question 10

This is also known as differential interface contrast (DIC)

Answer 10

Phase contrast microscopy

Question 11

Produce 3d images, caused by short depth of field and high resolution.

Answer 11

Phase contrast microscopy

Question 12

Uses UV light to excite the molecules and emits light.

Answer 12

Florescent microscopy

Question 13

What microscopy uses DAPI dye (46,6-diamidino phenylindole) that stains DNA dark blue.

Answer 13

Florescent microscopy

Question 14

Uses laser to excite florescent chemical dyes, and computer reconstruct the image with higher reso.

Answer 14

Confocal laser microscopy

Question 15

Different cuts can lead to creating a 3d model in this type of microscopy.

Answer 15

Confocal laser microscopy

Question 16

This microscopy automatically focus, aperture of light, and motorized stage, observed through a computer.

Answer 16

Digital microscopy

Question 17

This microscopy use to observed cell structure that are up to <2um.

Answer 17

Electron microscopy

Question 18

Microscopy to use observed cell structures up to molecular level 0.2 nm micrometer.

Answer 18

Transmission Electron Microscopy (TEM)

Question 19

This microscopy visualize the surface of the sample that are coated with metal coatings before observation.

Answer 19

Scanning electron microscopy (SEM)