Lesson 12: Culture Media Flashcards
are cultured in either liquid (broth) or on solid (agar) artificial media
bacteria and fungi
pioneered the use of agar as a base for culture media by Fannie eilshemius
robert koch
developed the petri dish (plate)
Richard petri
a container for solid culture media
petri dish (plate)
developed the pour plate method and was the first to use solid culture media for culture of bacteria
Koch
a nutrient material prepared for the growth of microorganisms in a laboratory
culture medium
when microbes are introduced into a culture medium to initiate growth growth
inoculum
microbes that grow and multiply in or on a culture medium
culture
process of growing microorganisms in culture by taking bacteria from the infection site
cultivation
a laboratory culture containing a single species of organism
pure culture
bacterial cultivation has three main purposes;
- to grow and isolate all bacteria
- infection and contaminants or colonizers
- identification and characterization
Ingredients of culture media. components of culture media
- water
- agar
- peptone
- yeast extract
- malt extract
- blood and serum
phases of growth medua
- liquid (broth) media
- solid (agar) media
- semisolid media
liquid (broth) media
- media nutrients are dissolved in water
solid (agar) media
-adding a solidifying agent to the nutrients and water
most common solidifying agent
agarose
petri dish containing the agar
agar
clones of cella originating from a single bacterial cell
colonies
a single colony or clone are considered “pure” nutrient agar
bacterial cultures
simplest and most common medium used routinely in diagnostic laboratories
2% agar to nutrient broth
other example of solid media
blood agar, chocolate agar, macConkey agar
semisolid media
-where agar is added to media in concentrations that are too low to solidify
is an example of a simple liquid medium that consist peptone, meat extract sodium chloride and water
nutrient broth
example of a simple solid medium
nutrient agar
a media that contain some ingredients of unknown chemical composition
complex media
the complex media contain;
water, carbon source, various salts, source of amino acids and nitrogen
special media
- enriched media
- enrichment media
- selective media
- indicator media
- transport media
- sugar media
enriched media
solid media that facilitate growth
enriched media
solid media that facilitate growth
enrichment media
are liquid media that stimulate the growth of certain bacteria
selective media
are solid that contain substances that inhibit the growth of all but a few bacteria
differential or indicator media
distinguish one microorganisms from another growing on the same media
transport media
used to maintain the viability of certain delicate organisms in clinical specimens
sugar media
contains 1% “sugar”
sugar media examples used for fermentation test
glucose, sucrose, lactose and mannitol
preserving bacterial cultures
refrigeration
deep freezing
lyophilization (freeze drying)
cold storage
drying methods
can be used for short term storage of bacterial cultures
refrigeration
is a process in which a pure culture of microbes is placed in a suspending liquid and quick frozen
deep freezing
a suspension of microbes is quickly frozen
lyophilization
common method of preserving stains of bacteria
cold storage
drying suspensions of bacteria for preservation purposes have been developed
drying methods
sterilizing agent with very high penetrating power
radiations
remove contaminating Microorganisms from solution
filtration
use of high-frequency sound waves beyond the sensitivity of the human ear
sonication
most dependable method of sterilization
heat
natural.method in sterilization of water in tanks
sunlight
process of inactivating microorganisms by direct exposure to chemical and physical agents
disinfection
are products that destroy or inhibit the growth of microy
disinfectants
products that destroy or inhibit the growth of microorganisms in or on living tissue
antiseptics
types of disinfectants
phenolic compounds
Halogens
alcohols
aldehydes
gases
surface active agents
oxidizing agent
dyes
heavy metals
acids alkalis
