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General Veterinary Microbiology > Lesson 12: Culture Media > Flashcards

Lesson 12: Culture Media Flashcards

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Biology 101 flashcards
1
Q

are cultured in either liquid (broth) or on solid (agar) artificial media

A

bacteria and fungi

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2
Q

pioneered the use of agar as a base for culture media by Fannie eilshemius

A

robert koch

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3
Q

developed the petri dish (plate)

A

Richard petri

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4
Q

a container for solid culture media

A

petri dish (plate)

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5
Q

developed the pour plate method and was the first to use solid culture media for culture of bacteria

A

Koch

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6
Q

a nutrient material prepared for the growth of microorganisms in a laboratory

A

culture medium

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7
Q

when microbes are introduced into a culture medium to initiate growth growth

A

inoculum

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8
Q

microbes that grow and multiply in or on a culture medium

A

culture

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9
Q

process of growing microorganisms in culture by taking bacteria from the infection site

A

cultivation

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10
Q

a laboratory culture containing a single species of organism

A

pure culture

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11
Q

bacterial cultivation has three main purposes;

A
  1. to grow and isolate all bacteria
  2. infection and contaminants or colonizers
  3. identification and characterization
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12
Q

Ingredients of culture media. components of culture media

A
  1. water
  2. agar
  3. peptone
  4. yeast extract
  5. malt extract
  6. blood and serum
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13
Q

phases of growth medua

A
  1. liquid (broth) media
  2. solid (agar) media
  3. semisolid media
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14
Q

liquid (broth) media

A
  • media nutrients are dissolved in water
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15
Q

solid (agar) media

A

-adding a solidifying agent to the nutrients and water

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16
Q

most common solidifying agent

A

agarose

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17
Q

petri dish containing the agar

A

agar

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18
Q

clones of cella originating from a single bacterial cell

A

colonies

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19
Q

a single colony or clone are considered “pure” nutrient agar

A

bacterial cultures

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20
Q

simplest and most common medium used routinely in diagnostic laboratories

A

2% agar to nutrient broth

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21
Q

other example of solid media

A

blood agar, chocolate agar, macConkey agar

22
Q

semisolid media

A

-where agar is added to media in concentrations that are too low to solidify

23
Q

is an example of a simple liquid medium that consist peptone, meat extract sodium chloride and water

A

nutrient broth

24
Q

example of a simple solid medium

A

nutrient agar

25
Q

a media that contain some ingredients of unknown chemical composition

A

complex media

26
Q

the complex media contain;

A

water, carbon source, various salts, source of amino acids and nitrogen

27
Q

special media

A
  1. enriched media
  2. enrichment media
  3. selective media
  4. indicator media
  5. transport media
  6. sugar media
28
Q

enriched media

A

solid media that facilitate growth

29
Q

enriched media

A

solid media that facilitate growth

30
Q

enrichment media

A

are liquid media that stimulate the growth of certain bacteria

31
Q

selective media

A

are solid that contain substances that inhibit the growth of all but a few bacteria

32
Q

differential or indicator media

A

distinguish one microorganisms from another growing on the same media

33
Q

transport media

A

used to maintain the viability of certain delicate organisms in clinical specimens

34
Q

sugar media

A

contains 1% “sugar”

35
Q

sugar media examples used for fermentation test

A

glucose, sucrose, lactose and mannitol

36
Q

preserving bacterial cultures

A

refrigeration
deep freezing
lyophilization (freeze drying)
cold storage
drying methods

37
Q

can be used for short term storage of bacterial cultures

A

refrigeration

38
Q

is a process in which a pure culture of microbes is placed in a suspending liquid and quick frozen

A

deep freezing

39
Q

a suspension of microbes is quickly frozen

A

lyophilization

40
Q

common method of preserving stains of bacteria

A

cold storage

41
Q

drying suspensions of bacteria for preservation purposes have been developed

A

drying methods

42
Q

sterilizing agent with very high penetrating power

A

radiations

43
Q

remove contaminating Microorganisms from solution

A

filtration

44
Q

use of high-frequency sound waves beyond the sensitivity of the human ear

A

sonication

45
Q

most dependable method of sterilization

A

heat

46
Q

natural.method in sterilization of water in tanks

A

sunlight

47
Q

process of inactivating microorganisms by direct exposure to chemical and physical agents

A

disinfection

48
Q

are products that destroy or inhibit the growth of microy

A

disinfectants

49
Q

products that destroy or inhibit the growth of microorganisms in or on living tissue

A

antiseptics

50
Q

types of disinfectants

A

phenolic compounds
Halogens
alcohols
aldehydes
gases
surface active agents
oxidizing agent
dyes
heavy metals
acids alkalis

General Veterinary Microbiology (17 decks)

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