Lesson 11: Aseptic Technique

Question 1

main objective of aseptic technique

Answer 1

eliminate contaminants from work surfaces

Question 2

elements of aseptic environment

Answer 2

quiet area
work surface
personal hygiene
reagents and media
cultures

Question 3

sterile handling

Answer 3

swabbing
capping
flaming
handling bottles and flasks
pipetting
pouring

Question 4

an equipment that protect work surface from dust and contamination by a constant, stable flow of filtered air passing over the work surface

Answer 4

safety cabinets (laminar flow)

Question 5

two types of safety cabinets

Answer 5

horizontal - air blows from the side facing you
vertical - air blows down from the top

Question 6

major source of contamination

Answer 6

humidified incubators

Question 7

incubators should be regularly cleaned with detergent and then swabbed with how many percent of alcohol?

Answer 7

70% alcohol

Question 8

essence of good sterile technique embodies many of the principles of standard good laboratory practice

Answer 8

standard procedure

Question 9

important that every new staff introduced into the sterile area

Answer 9

training

Question 10

contamination can be avoided by?

Answer 10

1. regular checking culture
2. avoidance of use antibiotics
3. always sterile reagents and materials
4. exclusive use of media
5. strict observation of sterile technique