Lecture 9

Question 1

cRNA refers to :

Answer 1

Crispr RNA

Question 2

The interspaces between the short palindromic repeats in CRISPR regions of bacterial genomes are made up of sequences from:

Answer 2

Bacteriophages

Question 3

The CRISPR system is found in____
?____
and uses___?____ and Cas to destroy foreign DNA.

Answer 3

bacteria; guide RNAs

Question 4

tracRNA has been coupled to____ to produce guide RNA

Answer 4

crRNA

Question 5

True or false
Class I CAS proteins capture: DNA that the cell has not with previously encountered

Answer 5

True

Question 6

gene-editing technique was used to create a disease model for Rett Syndrome in macaques by altering the
MEC2 gene
What method would be considered the easiest and least costly to undertake this?

Answer 6

CRISPR

Question 7

True or false
In CRISPR technology, homology-directed repair is applied when attempting to replace a faulty gene with a healthy version.

Answer 7

True

Question 8

What’s the PAM recognition sequence?

Answer 8

NGG

Question 9

PAM is an acronym for:

Answer 9

Protospacer adjacent motif

Question 10

True or false

CAS genes make helicases and endonucleases

Answer 10

True

Question 11

Using CRISPR, bacteria can prevent important memorized foreign sequences in their genome from being destroyed by ensuring that they lack a____ sequence

Answer 11

PAM