Lecture 4 and 5

Question 1

If not using a scientific calculator, to calculate “n” in the formula A = Ao x Pn
Correct
I should change the formula around as follows:

Answer 1

(log(A/Ao) )/logP= n

Question 2

What does “A” represent in the Formula:
A = Ao X P

Answer 2

Yield after PCR

Question 3

What does “n” represent in the Formula:
A = A X Pn

Answer 3

Cycle Number

Question 4

A genome is 4 x 10% bp in size. In a PCR vial, a starting quantity of 20 ng genomic DNA is included. The target amplicon within this genome is a 2kbp (two kilobase pair) gene. After 35 cycles of PCR, the yield of amplicon is 200 ng. What is the efficiency of the PCR cycle?

Answer 4

1.51

Question 5

When PCR efficiency is low, the PCR amplicon…

Answer 5

may not form a visible band on a gel

Question 6

What does “P” represent in the Formula:
A = Ao X Pn

Answer 6

Efficiency of the PCR reaction

Question 7

A single nucleotide weighs

Answer 7

330 Daltons

Question 8

The molecular weight of an individual atom or an individual molecule is described using which weight unit

Answer 8

Daltons

Question 9

A bp is a loose term that molecular biologist use to describe a

Answer 9

a nucleotide on one strand of double-stranded DNA that is hydrogen bonded to a nucleotide on the complementary strand

Question 10

a single molecule of water weighs 18 Daltons, then a mole of water weighs

Answer 10

18 grams

Question 11

What does “Ao” represent in the Formula:
A = Ao X Pn

Answer 11

Starting amount of amplicon pre PCR