Lecture 6

Question 1

True or false
During Illumina sequencing, all four capped fluorophore nucleotides are added together at every cycling

Answer 1

True

Question 2

Portable on-site environmental sequencing can be undertaken by what?

Answer 2

Oxford Nanopore Sequencing

Question 3

True or false
The Illumina Adapter referred to as as index sequence is the equivalent of what is normally referred to as a barcode

Answer 3

True

Question 4

The oligonucleotides attached to the inner surface of Illumina flow cells are of two types. Collectively these oligos are referred to as…

Answer 4

Capture oligos

Question 5

The term “sequencing by synthesis” is applied to what sequencing technologies?

Answer 5

Sanger Dideoxy
Illumina
Pyrosequencing