Lecture 7

Question 1

siRNA is an abbreviated version of..

Answer 1

small interfering RNA

Question 2

The targets of a microRNA are usually

Answer 2

tissue-specific genes

Question 3

RNA interference is thought to have evolved to

Answer 3

defend against viruses

Question 4

Which component of the RNAi pathway confers inheritance of siRNA-induced silencing?

Answer 4

RdRP (RNA-directed RNA polymerase)

Question 5

Morpholino oligonucleotides have proved useful for

Answer 5

antisense-mediated inhibition of gene expression

Question 6

A target RNA-morpholino hybrid does not promote mRNA degradation because:

Answer 6

It is not a substrate for RNaseH

Question 7

True or false
miRNAs are found in plants but not in animals.

Answer 7

False

Question 8

RNA interference is the process of what?

Answer 8

gene silencing at the post-transcriptional level.

Question 9

MicroRNAs are processed by the enzymes_____ and_____ One strand of the microRNA is loaded into silencing complex named____ The microRNAs target mRNA for ____ or ____

Answer 9

Drosha, Dicer, RISC, mRNA degradation, translational repression

Question 10

The target mRNA for a particular miRNA is cleaved by

Answer 10

RISC

Question 11

The target mRNA for a particular miRNA is cleaved by

Answer 11

RISC

Question 12

True or false
The targets of a microRNA are usually tissue-specific genes

Answer 12

True

Question 13

Antisense oligonucleotides suppress gene expression relatively poorly, in part because of

Answer 13

inefficient delivery to cells

Insufficient target sequences

Question 14

True or false
Dicer is the component of the RNAi pathway that confers inheritance of siRNA-induced silencing?

Answer 14

False

Question 15

True or false
RNA interference is the process of gene silencing at the level of RNA splicing.

Answer 15

False