Lecture 8

Question 1

the Cre-lox system- The Cre recombinase will

Answer 1

excise DNA between two lox P sites

Question 2

When a gene has loxP on both sides it is said to be ______

Answer 2

floxed

Question 3

Cre recombinase is driven by a

Answer 3

tissue-specific promoter.

Question 4

Cre-lox system works best if the parent with the floxed gene is

Answer 4

a heterozygote, with the second copy of the gene inactive. This is due to the the Cre-lox system not being 100% effective

Question 5

_______ can be used as an inducer for the Cre system.

Answer 5

Tamoxifen

Question 6

When inducing Cre, an the system is activated by using a fusion of the Cre enzyme with the _____________

Answer 6

ligand-binding domain of the estrogen receptor

Question 7

Tet systems do not change _______

Answer 7

the DNA. This allows them to easily be turned on and off. Uses VP 16 as an activator

Question 8

Cre lox system can cause flipping of sister sdjscent homologues

Answer 8

cells will fluoresce different colors