Lecture 7: Cytoskeleton IV

Question 1

Name the three types of mitotic MT

Answer 1

-astral MT
-kinetochore MT
-Polar MT

Question 2

Astral MT function

Answer 2

connect the spindle to membrane to ensure the spindle position

Question 3

Kinetochore MT function

Answer 3

attach to kinetochore to pull chromosome apart

Question 4

Polar MT function

Answer 4

use kinesin motor to push the two spindle poles apart

Question 5

Describe FRAP

Answer 5

Fluorescence Recovery After Photobleaching
-helps determine how fast the GFP signal recovers at the site of photobleaching

Question 6

XMAP215

Answer 6

-stabilizes (+)
-promotes MT growth
-regulated by phosphorylation

Question 7

Name the drug used as an anti cancer drug

Answer 7

Taxol

Question 8

What does taxol do?

Answer 8

It binds to microtubules and inhibits microtubule depolymerization and dynamics- inhibits mitosis so the cancer cells will be killed due to mitotic effect

Question 9

What is tightly controlled during cell cycle?

Answer 9

centrosome number

Question 10

Why is it important to tightly control centrosome number?

Answer 10

Too many centrosomes:
-chromosome segregation defects
-genetic instability
-tumorigenesis

Question 11

What do centrosome abnormalities suggest

Answer 11

cancer

Question 12

Intermediate filaments support

Answer 12

nuclear structure (lamins)

Question 13

Lamin related disease

Answer 13

Hutchinson Gilford Progeria Syndrome (HGSP)

Question 14

List three things about IF

Answer 14

-dynamic
-assembly occurs spontaneously
-energy is required for disassembly

Question 15

Which phase does duplication happen?

Answer 15

S phase