Lecture 3: Mitochondria

Question 1

What does the movement of electrons down the ETC lead to?

Answer 1

the establishment of a electrochemical proton gradient

Question 2

What does the electrochemical proton gradient cause?

Answer 2

The generation of ATP through ATP synthase

Question 3

What does ATP synthesis require?

Answer 3

A proton impermeable inner mito membrane

Question 4

ATP synthase converts __ energy into _ energy to obtain ATP

Answer 4

chemical, mechanical

Question 5

How many distinct membranes do mitochondria contain?

Answer 5

2 with protein and lipid composition function

Question 6

What do mitochondria activities depend on?

Answer 6

protein and RNA import from the cytosol

Question 7

What is one mitochondrial disease?

Answer 7

Leigh syndrome

Question 8

What cause mitochondrial diseases?

Answer 8

mutations in the mitochondrial DNA ad/or nuclear DNA encoding protein that function in mitochondrias

Question 9

What does the severity of mitochondrial diseases depend on?

Answer 9

The % of mito with mutated DNA

Question 10

What is immunofluorescence?

Answer 10

Cells that are chemically fixed and stained with primary antibodies that recognize the antigen of interest and secondary antibodies that are coupled to fluorophores and that recognize primary antibody