Lecture 7 Flashcards
receptor mediated endocytosis
Cells take in macromolecules
endocytic pathway
- Membrane-bound compartments
- Early endosomes, Late endosomes and Lysosomes
early endosomes
-recycling and sorting
receptors release ligands at
low pH. change # of protons. conformational change of receptor allows release of ligand
Vacuolar ATPase (or proton pump)-
- ATP is hydrolyzed and changes structure. Protons going against gradient
- proton pump maintains low pH in lysosomes
- production of nutrients for cell
what are located in lysosomes?
acid hydrolases- only work at low pH.
Ph 4.5 in lysosome, 5.5 early endosome, 6.5 late endosome. Why diff pH if theres proton pump in all 3?
You have more proton pumps in different areas.
Binding of ligand to a receptor (LDL)-
- LDL (low density lipoprotein) binds and transports cholesterol to cells
- Atherosclerosis (plagues in bloodstream)
bad
LDL
LDL
HDL
Triglycerides
-too much in blood can lead to plaques. Carries cholesterol from liver to other tissues.
HDL - carries cholesterol back to liver where it is passed from body
Triglycerides - fat made in body (high levels signal problems)
cholesterol binds to
LDL, LDL bound to LDL receptor. Bound to adaptin and clathrin. Then you make clathrin coated pit then transported
ways it can get messed up
- cholesterol doesnt bind to LDL
- LDL doesnt bind to LDL receptor
- LDL receptor doesnt bind to adaptins
- cholesterol stays out of cells
YXX(PSI)
signal peptide for CCP
psi-hydrophobic aa
NPVY
in LDL receptor
Receptor recycles for additional rounds of endocytosis
lifespan 20 hours, can make the trip 700 times
transferrin
soluble protein that transports iron in blood
Transferrin w iron-
transferrin receptor
In the blood stream if you have transferrin without iron-
APOtransferrin. why doesnt it bind? structure isnt correct