Lecture 4 Preparation of Specimens for Microscopic Examination

Question 1

Used to observe
living organisms’ actual shape and motility?

Answer 1

wet mount preparation

Question 2

Uses ordinary glass
slide and cover slip.

Answer 2

simple wet mount

Question 3

Uses
depression slide and cover slip.

Answer 3

hanging drop technique

Question 4

Types of wet mount preparation.

Answer 4

-simple wet mount
- hanging drop technique

Question 5

uses stains/dyes;
microorganisms observed are in their dead state-
enables us to:
-make transparent and semi-transparent objects
visible
-observe the size and shape of microorganisms
-detect the presence of various internal and
external structures
-detect physical and chemical reactions

Answer 5

stained smear preparation

Question 6

Types of preparation?

Answer 6

wet mount preparation
stained smear preparation

Question 7

-A thin film of a microbe solution on a
slide,
-usually fixed to attach microbes to the
slide and kill microbes

Answer 7

Smear

Question 8

Types of dyes/stains?

Answer 8

Basic and acid dyes

Question 9

Types of dyes where the positively-
charged. Chromophore is a
cation

Answer 9

Basic dyes

Question 10

Examples of basic dyes?

Answer 10

methylene blue, crystal
violet, safranin

Question 11

Types of Dyes/Stains where they are negatively
charged.

Answer 11

congo red, acid fuchsin,
eosin. Chromophore is an
anion

Question 12

What are the Basic Types of Staining
Procedures?

Answer 12

Positive staining
Negative staining

Question 13

Basic Types of Staining
Procedures where dye
actually sticks to the specimen
and gives it color.

Answer 13

Positive staining

Question 14

Basic Types of Staining
Procedures where negatively
charged
e.g. congo red, acid fuchsin,
eosin. Chromophore is an
anion

Answer 14

Negative staining

Question 15

Kinds of Positive
Staining Methods

Answer 15

Simple staining
Differential staining

Question 16

Kinds of Positive
Staining Methods where involves application of a
single stain/dye to a fixed
smear; e.g. methylene blue
staining of bacteria
-cells exhibit more or
less the same color

Answer 16

Simple staining

Question 17

Kinds of Positive
Staining Methods where it involves application of 2 or
more stains/dyes to a fixed smear;

Answer 17

Differential staining

Question 18

Basic Types of Staining
Procedures where qstained cells exhibit different colors (e.g.
methylene blue + safranin in staining of cheek cells
(stained pink) and Diplococcus salivarious (stained
blue)