lecture 33 - oxidative phosphorylation Flashcards
what are the two energetic gradients that are a result of the proton gradient across the inner mitochondrial membrane
- chemical gradient of pH gradient due to different H+ concentrations on either side of the membrane
- electrical gradient due to the charge difference across the membrane (positive in the inter-membrane space, negative in the matrix)
what is the electrochemical gradient known as
the proton motive force (pmf)
what can not occur in the ETC if there is no membrane to create a proton gradient
ATP synthesis
what happens if you take DNP
- ETC functions
- No ATP is made
how does DNP cause weight loss
the cells needs to make ATP and if the fuel molecules aren’t providing that, they will feed more and more into the cell to try and make it = increase use of fuel molecules = weight loss
what happens to energy stored in the gradient when you take DNP
normally = used to make ATP
when you take DNP = gets converted to heat (which is why people have died)
where is F1 in the F1Fo-ATP synthase
in the matrix
where is Fo in the F1Fo-ATP synthase
in the inner mitochondrial membrane
what do the rotor subunit do in the F1Fo-ATP synthase
the rotor subunits turn
what do the stator subunits do in the F1Fo-ATP synthase
the stator subunits do not turn
what drives the rotor movement in F1Fo-ATP synthase
proton flow
what does rotor movement cause in F1Fo-ATP synthase
causes conformational change in the stator that drives ATP synthesis
what does the inter-membrane space have a higher content of and what does this favour
higher content of protons, energetically favourable for the protons to be going from the inter-membrane space into the matrix (ATP synthase provides the pathway for this)
protons enter the channel and interact with what in the F1Fo-ATP synthase
interact with the binding pockets on the c subunits (causes it to start turning)
where does ATP synthesis occur within F1Fo-ATP synthase
the pairs of alpha and beta subunits
