lecture 30 - the final oxidation : the citric acid cycle Flashcards
where does the citric acid cycle occur
in the mitochondrial matrix
where does B oxidation occur
in the mitochondrial matrix
where does the electron transport chain occur
in the inner membrane
what enters and leaves the citric acid cycle
2 C enters as acetyl-CoA
2 C out as 2 x CO2 (oxidation complete)
what energy is captured from the citric acid cycle
1 ATP
3 NADH and 1 FADH2
what reactions produce acetyl-CoA for the citric acid cycle
glycolysis (aerobic, PHD reaction) and B oxidation
what are the two stages of the citric acid cycle
- release of C
- regeneration of the starting molecule
where does the energy come from to attach acetyl-CoA to 4 oxaloacetate to produce 6 C citrate
hydrolysis of CoA from acetyl-CoA
energetically favourable = the energy to add the two carbons is from coming from taking the CoA off
in the citric acid cycle what does the rearrangement of citrate to isocitrate make the molecule susceptible
decarboxylation
what are the consequences of inhibiting the citric acid cycle
- increased acetyl-CoA
- decreased reduced enzyme
- less ATP
what type of reaction is removal of the first carbon in the citric acid cycle and how many steps are involved, what form is energy captured in
oxidative decarboxylation, 2 steps
energy captured = NADH
what type of reaction is the removal of the second carbon in the citric acid cycle and what reaction is it similar to
a second oxidative decarboxylation reaction
- very similar to the pyruvate dehydrogenase reaction
how many carbons are removed in total from the citric acid cycle
2 carbons removed
what is the second phase of the citric acid doing
regenerating oxaloacetate and capturing more energy
what is GTP the energy equivalent of
ATP
