Lecture 3: Gel Electrophoresis Flashcards
1
Q
Gel electrophoresis
A
Method to seperate DNA molecules based on their size using an electric field to move molecules through a gel matrix
2
Q
The gel used:
A
Agarose polymer: forms complex matric of pores
3
Q
Negative charge is applied to
A
the top of the gel where the DNA is added to the wells
4
Q
Positive charge is applied to the
A
bottom of the gel
5
Q
DNA charge is… because…
A
negative because of the phosphates
6
Q
Small DNA molecules will travel
A
quickly through the gel matrix
7
Q
Large DNA molecules will travel
A
slowly through the gel matrix (Stuck in pores)
8
Q
Molecular ladder
A
Contains DNA pieces of know sizes to help determine the sizes of your DNA samples
9
Q
DNA travels at a rate…. this is why the spacing…
A
- that is inversely proportional to the log of its size
- the space between 2000->1000 isnt the same as 200->100 because DNA travels at a rate inversely propotional to the log of its size
10
Q
Plasmids can be found in 3 different conformations
A
- supercoiled
- circular relaxec (nicked)
- Linear
