Lecture 2.3 Flashcards
To answer the question whether there is systemic inflammation, what two tests can be done?
- Measure SED rate (negative blood cells bind to positive acute phase proteins)
- Measure the amount of C-reactive protein
Which type of immunofluorescence is used for looking for something on patient tissue (direct/indirect)
Direct
Which type of immunofluorescence is used for looking for something on patient serum (direct/indirect)
Indirect
Type of screening that uses indirect immunofluorescence on Hep 2 cells
Anti nuclear antibody
A false positive for an anti nuclear antibody would be (homogeneous/ speckled) pattern
speckled
This type of screening is used to detect antibodies in lupus and can have false positives in syphilis
antiphospholipid antibody
Cytoplasmic anti-neutrophil cytoplasmic antibodies (C-ANCA) is specific for this immunoassay antigen (alcohols stain for cytoplasm)
proteinase 3
Perinuclear-ANCA is specific for this immunoassay antigen (alcohol stains for nucleus)
MPO
What are two tests for rheumatoid arthritis?
- Rheumatoid factor (IgM attached to Fc portion of IgG)
2. Anti-CCP
Describe the direct anti-globulin test
Red blood cells are dropped into antiglobulin reagents and then look for reaction (detects antibody of complement on RBC
Describe the indirect anti-globulin test
Place serum into tubes containing RBC and other antigens and look for agglutination or hemolysis (detects antibodies in the serum)
What is the most positively charged antibody
IgG
What tests can be used to detect hypergloubulinemia or abnormal globulins present
Serum electrophoresis, Immunofixation, counting immunoglobulin levels
Do serum levels of complement increase of decrease with complement activation secondary to immune complex disease (Type 3 hypersensitivity)?
Decrease, should be in tissues
