Lecture 21 - Biotechnology in use Flashcards
Recombinant DNA technology
Therapeutic products
Diagnosis
GM Products
Energy applications
Many things influence the host choice for protein production
what are they?
Financial reasons (speed, cost) Biochemical reasons (Glycosylation, Folding)
Insulin
PTM? (post transitional modifications)
Best produced in?
No
In bacteria (cost) and Mammalian cells
EPO
PTM? (post transitional modifications)
Best produced in?
Yes Glycosylation
Mammalian cells
Antithrombin
PTM? (post transitional modifications)
Best produced in?
Yes Carboxylation
In transgenic animals
not bacterial or mammalian cells
How can we make new proteins?
Use recombinant DNA technology
Mix and match different proteins or different genes from different sources
How can we provide a permanent treatment?
Use patients as the host for protein production!
How can Plasmids be carried into patients?
using viral vectors = Gene therapy
Recombinant protein
Insulin Factor IX EPO INF Antibodies
Type 1 diabetes Problem
Beta cells in pancreas destroyed
= autoantibodies recognise proteins on beta cell surface
= no insulin produced.
Type 1 diabetes solution
Use gene therapy to Turn liver cells into insulin making machines.
Gene therapy could permanently treat
Type 1 diabetes
In Plasmid:
Glucose response promoter
Pre pro insulin cDNA
Gene editing
CRISPR-Cas
CRISPR-Cas
Use DNA to correct the genome (return functional copy of the gene)
Different protein factories are required
depending on ion complexity of protein you want to produce.
